Field resistance against potato virus Y infection using natural and genetically engineered resistance genes

Commercial tobacco cultivars BB16 (burley) and PBD6 (dark air cured) were transformed with the coat protein gene of lettuce mosaic potyvirus (LMV). Transgenic BB16 plants showed resistance to potato virus Y (PVY) infection, against the necrotic strain PVY-N Versailles, as well as the resistance brea...

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Bibliographic Details
Published in:European journal of plant pathology 1998-05, Vol.104 (4), p.377-382
Main Authors: Dinant, S. (Station de Pathologie Vegetale INRA, Versailles (France).), Kusiak, C, Cailleteau, B, Verrier, J.L, Chupeau, M.C, Chupeau, Y, Le, T.A.H, Delon, R, Albouy, J
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Coat protein
Crop diseases
Cultivars
Disease resistance
Fundamental and applied biological sciences. Psychology
GENETIC ENGINEERING
GENETIC RESISTANCE
Genetics and breeding of economic plants
GENIE GENETIQUE
INGENIERIA GENETICA
Life Sciences
NICOTIANA TABACUM
Pest resistance
Plant pathogens
Plant virus diseases
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
POTATO Y POTYVIRUS
Potatoes
POTYVIRUS Y DE LA PATATA
POTYVIRUS Y POMME DE TERRE
Proteins
RESISTANCE GENETIQUE
RESISTENCIA GENETICA
Tobacco
TRANSGENIC PLANTS
Varietal selection. Specialized plant breeding, plant breeding aims
Viruses
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Summary:Commercial tobacco cultivars BB16 (burley) and PBD6 (dark air cured) were transformed with the coat protein gene of lettuce mosaic potyvirus (LMV). Transgenic BB16 plants showed resistance to potato virus Y (PVY) infection, against the necrotic strain PVY-N Versailles, as well as the resistance breaking necrotic strain PVY-N 107. Transgenic PBD6, which carries the recessive ‘va’ gene conferring resistance to PVY, was also protected against PVY-N 107. In the progenies of most BB16 and PBD6 transformants, 45% to 100% of the inoculated plants were immune to PVY infection. The rest of the plants were tolerant, with atypical attenuated non necrotic symptoms and reduced virus accumulation. No recovery was observed in the tolerant plants, which stably expressed detectable level of LMV coat protein. This suggested a protein-mediated mechanism of heterologous protection.
ISSN:0929-1873
1573-8469
DOI:10.1023/A:1008079003061