Purification and cloning of the mitochondrial branched-chain amino acid aminotransferase from sheep placenta

• Published in: European Journal of Biochemistry 1999-01, Vol.259 (1/2), p.104-111
• Main Authors: Faure, M, Glomot, F, Bledsoe, R, Hutson, S, Papet, I
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; animal physiology; animal reproduction; Animals; Base Sequence; branched-chain-amino-acid aminotransferase; branched‐chain amino acids; cDNA; Cloning, Molecular; DNA, Complementary - genetics; Female; Gene Expression; Isoenzymes - analysis; Life Sciences; Mitochondria - enzymology; Molecular Sequence Data; Muscle, Skeletal - enzymology; Placenta - enzymology; Pregnancy; protein purification; Sequence Homology, Amino Acid; Sheep; Tissue Distribution; Transaminases - analysis; Transaminases - genetics; Transaminases - isolation & purification
• ISSN: 0014-2956; 1432-1033; 1432-1327
• DOI: 10.1046/j.1432-1327.1999.00009.x

This paper presents the first purification of the mitochondrial branched‐chain amino acid aminotransferase (BCATm) from sheep placenta. It is a homodimer with an apparent subunit molecular mass of 41 kDa. The enzyme differs from those of the rat and human as it appears to form at least one intermolecular disulfide bond. The sheep BCATm cDNA (1.4 kb) encodes a mature polypeptide of 366 amino acids with a calculated molecular mass of 41 329 Da and a partial mitochondrial targeting sequence of seven amino acids. The sheep BCATm sequence shares higher identity with other mammalian BCATm isoenzymes (82–85%) than with the cytosolic isoenzymes (60%). By Northern blot analysis, a message of 1.7 kb was detected in sheep placenta and skeletal muscle. Measurements of BCAT activity, mRNA and BCATm protein in sheep placenta and skeletal muscle revealed that BCATm is the sole BCAT isoenzyme expressed in placenta, whereas it contributes 57 and 71% of the BCAT activity in tensor fascia latae and masseter muscles from weaned lambs respectively. Skeletal muscle, the main site of branched‐chain amino acid transamination, exhibits significantly lower BCAT activity in sheep than in rat. Our results suggest that the low BCATm mRNA level probably accounts for the low BCAT activity in sheep skeletal muscle, and that the metabolic scheme for branched‐chain amino acid catabolism is specific to each species.