In vivo nuclear magnetic resonance study of citrate metabolism in Propionibacterium freudenreichii subsp. shermanii

Citrate metabolism by resting cells of Propionibacterium freudenreichii subsp. shermanii was investigated. In vivo13C nuclear magnetic resonance spectroscopy was used to study the pathway of citrate breakdown and to probe its utilization, non-invasively, in living cell suspensions. [2,4-13C]citrate...

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Bibliographic Details
Published in:Journal of dairy research 1998-08, Vol.65 (3), p.503-514
Main Authors: DEBORDE, CATHERINE, ROLIN, DOMINIQUE B., BONDON, ARNAUD, DE CERTAINES, JACQUES D., BOYAVAL, PATRICK
Format: Article
Language:English
Subjects:
Agricultural sciences
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Food industries
Fundamental and applied biological sciences. Psychology
Life Sciences
Milk and cheese industries. Ice creams
Mission oriented research
Physiology and metabolism
Propionibacterium freudenreichii shermanii
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Summary:Citrate metabolism by resting cells of Propionibacterium freudenreichii subsp. shermanii was investigated. In vivo13C nuclear magnetic resonance spectroscopy was used to study the pathway of citrate breakdown and to probe its utilization, non-invasively, in living cell suspensions. [2,4-13C]citrate was metabolized by resting cells to glutamate labelled in positions 2 and 4. In the presence of lactate or pyruvate, its rate of consumption was faster, but it was still converted to glutamate. No catabolic pathway other than the first third of a turn of the tricarboxylic acid cycle was used by Prop. freudenreichii subsp. shermanii to degrade citrate.
ISSN:0022-0299
1469-7629
DOI:10.1017/S0022029998002878