The efficiency of different IRESs (internal ribosomes entry site) in monocistronic mRNAS

The IRES from poliovirus and from encephalomyocarditis virus (EMCV) added between the cap and the AUG initiator codon were strong inhibitors of chloramphenicol acetyltransferase gene expression in three different cell types. The poliovirus IRES also inhibited bGH (bovine growth hormone) cDNA express...

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Bibliographic Details
Published in:Molecular biology reports 2000-03, Vol.27 (1), p.21-26
Main Authors: Attal, J, Théron, M C, Rival, S, Puissant, C, Houdebine, L M
Format: Article
Language:English
Subjects:
5' Untranslated Regions
Animals
Carrier Proteins - genetics
Cell Line
Cells
Chloramphenicol O-Acetyltransferase - genetics
Chloramphenicol O-Acetyltransferase - metabolism
CHO Cells
Codon, Initiator
Cricetinae
Encephalomyocarditis virus
Encephalomyocarditis virus - genetics
Gene expression
Genes, Reporter
Heat-Shock Proteins
HeLa Cells
Human T-lymphotropic virus 1
Human T-lymphotropic virus 1 - genetics
Humans
immunoglobulin heavy chain-binding protein
internal ribosome entry site
Life Sciences
Milk Proteins - genetics
Molecular Chaperones - genetics
Poliovirus
Poliovirus - genetics
Promoter Regions, Genetic
Protein Biosynthesis
Proteins
Rabbits
RNA Caps
RNA, Messenger
Rous sarcoma virus
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Summary:The IRES from poliovirus and from encephalomyocarditis virus (EMCV) added between the cap and the AUG initiator codon were strong inhibitors of chloramphenicol acetyltransferase gene expression in three different cell types. The poliovirus IRES also inhibited bGH (bovine growth hormone) cDNA expression in the HC11 mammary cell line when added between the rabbit whey acidic gene promoter and the cDNA whereas the HTLV-1 IRES showed a stimulatory effect in the same situation. RNA stem loops were added before HTLV-1 (SUR) and the BiP (Immunoglobulin heavy-chain Binding Protein) IRESs followed by the firefly luciferase gene under the control of Rous sarcoma virus (RSV) promoter. The RNA loops abolished the expression of the reporter gene almost completely. These data suggest that the different IRESs may favour or inhibit translation of monocistronic mRNA.
ISSN:0301-4851
1573-4978
DOI:10.1023/A:1007084730470