A new small RNA virus persistently infecting an established cell line of Galleria mellonella, induced by a heterologous infection

A persistent infection in a Galleria mellonella cell line was revealed when infected with a maize stem borer picorna-like virus isolated on Sesamia cretica (MSBV). The new virus, completely different from the MSBV, is designated as G. mellonella cell line virus (GmclV), induces spectacular cytopathi...

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Bibliographic Details
Published in:Journal of invertebrate pathology 1997-01, Vol.69 (1), p.7-13
Main Authors: Lery, X. (Entomovirology Laboratory ORSTOM, Cairo, Egypt.), Fediere, G, Taha, A, Salah, M, Giannotti, J
Format: Article
Language:English
Subjects:
Animal biology
Animals
Biological and medical sciences
Cell Line
CULTIVO DE CELULAS
CULTURE DE CELLULE
Cytopathogenic Effect, Viral
Enzyme-Linked Immunosorbent Assay
Female
Fundamental and applied biological sciences. Psychology
GALLERIA MELLONELLA
INFECCIONES LATENTES
INFECTION LATENTE
Insect Viruses
Insecta
Invertebrate Zoology
Invertebrates
Lepidoptera - virology
Life Sciences
Microscopy, Electron
Ovary - cytology
Ovary - virology
Pathology
PATOGENICIDAD
PICORNAVIRIDAE
Picornaviridae - isolation & purification
Picornaviridae Infections - virology
POUVOIR PATHOGENE
RNA Viruses
VIRUS DE INVERTEBRADOS
VIRUS DES INVERTEBRES
Virus Latency
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Description
Summary:A persistent infection in a Galleria mellonella cell line was revealed when infected with a maize stem borer picorna-like virus isolated on Sesamia cretica (MSBV). The new virus, completely different from the MSBV, is designated as G. mellonella cell line virus (GmclV), induces spectacular cytopathic effects, and is also considered efficient in vivo. The GmclV is a 29-nm-diameter isometric virus, with single-strand RNA of 2.9 x 10(6) Da molecular weight with a poly(A) tract. Its capsid is constituted of only two major polypeptides, of 34,500 and 32,500 Da, and no minor bands could be detected. The characteristics of the GmclV do not permit us to classify it with assurance. Even though it has not yet been identified as a picornavirus, it can be classified in the small RNA virus group of the Picornaviridae. G. mellonella represents a very interesting model, owing to the fact that two different persistent viruses belonging to the same family were isolated in vivo and in vitro, to further the understanding of the general phenomenon of persistency and induction
ISSN:0022-2011
1096-0805
DOI:10.1006/jipa.1996.4617