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Characterization of the cryptogein binding sites on plant plasma membranes
Cryptogein is a 98-amino acid proteinaceous elicitor of tobacco defense reactions. Specific binding of cryptogein to high affinity binding sites on tobacco plasma membranes has been previously reported ( K d = 2 n m ; number of binding sites: 220 fmol/mg of protein). In this study, biochemical chara...
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Published in: | The Journal of biological chemistry 1999-12, Vol.274 (49), p.34699-34705 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Cryptogein is a 98-amino acid proteinaceous elicitor of tobacco defense reactions. Specific binding of cryptogein to high
affinity binding sites on tobacco plasma membranes has been previously reported ( K
d = 2 n m ; number of binding sites: 220 fmol/mg of protein). In this study, biochemical characterization of cryptogein binding sites
reveals that they correspond to a plasma membrane glycoprotein(s) with an N -linked carbohydrate moiety, which is involved in cryptogein binding. Radiation inactivation experiments performed on tobacco
plasma membrane preparations indicated that cryptogein bound specifically to a plasma membrane component with an apparent
functional molecular mass of 193 kDa. Moreover, using the homobifunctional cross-linking reagent disuccinimidyl suberate and
tobacco plasma membranes incubated with 125 I-cryptogein, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, two 125 I-cryptogein linked N -glycoproteins of about 162 and 50 kDa. Similar results were obtained using Arabidopsis thaliana and Acer pseudoplatanus plasma membrane preparations, whereas cryptogein did not induce any effects on the corresponding cell suspensions. These
results suggest that either cryptogein binds to nonfunctional binding sites, homologues to those present in tobacco plasma
membranes, or that a protein involved in signal transduction after cryptogein recognition is absent or inactive in both A. pseudoplatanus and A. thaliana . |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.274.49.34699 |