Effect of nitrate and incubation conditions on the production of catalase and nitrate reductase by staphylococci

The objective of this work was to study the production of catalase and nitrate reductase by staphylococci in order to understand their role in lipid oxidation during sausage manufacturing. Catalase and nitrate reductase were measured in resting cells and supernatants of staphylococci grown in differ...

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Bibliographic Details
Published in:International journal of food microbiology 1999-11, Vol.52 (1-2), p.47-56
Main Authors: TALON, R, WALTER, D, CHARTIER, S, BARRIERE, C, MONTEL, M. C
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Catalase - analysis
Catalase - biosynthesis
Cheese - microbiology
Colorimetry
Food industries
Food Microbiology
Fundamental and applied biological sciences. Psychology
Life Sciences
Lipid Metabolism
Meat and meat product industries
Meat Products - microbiology
Microbiology and Parasitology
Nitrate Reductase
Nitrate Reductases - analysis
Nitrate Reductases - biosynthesis
Nitrates - pharmacology
Nitrites - analysis
Oxidation-Reduction
Staphylococcal Infections - prevention & control
Staphylococcus
Staphylococcus - drug effects
Staphylococcus - enzymology
Staphylococcus - growth & development
Swine
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Summary:The objective of this work was to study the production of catalase and nitrate reductase by staphylococci in order to understand their role in lipid oxidation during sausage manufacturing. Catalase and nitrate reductase were measured in resting cells and supernatants of staphylococci grown in different conditions. All staphylococci (except S. warneri) synthetized nitrate reductase. In static condition, the synthesis was maximal during exponential growth phase, whereas in shaking condition, the synthesis was maximal at the beginning of stationary phase. The production of nitrate reductase was increased in presence of nitrate, this effect was particularly important for the two S. carnosus strains which exhibited the highest activity. For all staphylococci, the production of catalase was maximal at the end of the exponential growth phase. The lowest amount of catalase was produced by S. warneri and the highest by S. carnosus. Only S. xylosus 873 and S. saprophyticus 852 released high amounts of catalase in the supernatant growth. Staphylococci produced higher amounts of catalase in shaking conditions. Addition of nitrate in the growth media favoured the synthesis of catalase, with a pronounced effect for S. carnosus. Nitrate also favoured the release of catalase.
ISSN:0168-1605
1879-3460
DOI:10.1016/S0168-1605(99)00127-0