Soluble acid invertase activity in leaves is independent of species differences in leaf carbohydrates, diurnal sugar profiles and paths of phloem loading

• Published in: The New phytologist 1998-06, Vol.139 (2), p.283-292
• Main Authors: KINGSTON-SMITH, ALISON H., GALTIER, NATALIE, POLLOCK, CHRISTOPHER J., FOYER, CHRISTINE H.
• Format: Article
• Language: English
• Subjects: Acid invertase; Agronomy. Soil science and plant productions; Barley; Biological and medical sciences; Carbohydrate metabolism; Corn; Economic plant physiology; Fundamental and applied biological sciences. Psychology; hexose; Hexoses; Leaves; Life Sciences; Metabolism; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; Phloem loading; Photosynthesis, respiration. Anabolism, catabolism; Phytopathology and phytopharmacy; Plant physiology; Plant physiology and development; Plants; starch; Starches; sucrose; Vegetal Biology
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1046/j.1469-8137.1998.00198.x

Leaf sucrose, starch, hexose and maximum extractable soluble acid invertase activity were compared throughout the day in source leaves of 13 plant species chosen for their putative phloem-loading type (apoplastic or symplastic). Four species which represent the different phloem-loading types (tomato, barley, maize and Fuchsia) were studied in detail. Using this information we wished to determine whether a positive correlation between foliar carbohydrates and acid invertase activity exists in leaves from different species and, furthermore, whether this relationship is determined by phloem-loading type. Acid invertase activity was relatively constant throughout the day in all species. The extent of sucrose, hexose and starch accumulation and the sucrose: starch ratio measured at a given time were species-dependent. No correlations were found between foliar soluble acid invertase activity and the hexose, sucrose or starch content of the leaves in any of the species, regardless of phloem-loading type. The species examined could be divided into three distinct groups: (1) high sucrose, low invertase; (2) low sucrose, low invertase; and (3) low sucrose, high invertase. The absence of an inverse relationship between leaf sucrose, hexose or starch contents and endogenous soluble acid invertase suggests that this enzyme is not directly involved in carbon partitioning in leaves but serves an auxiliary function.