Mouse 5-HT2B Receptor-mediated Serotonin Trophic Functions

• Published in: Annals of the New York Academy of Sciences 1998-12, Vol.861 (1), p.67-73
• Main Authors: CHOI, D.-S., KELLERMANN, O., RICHARD, S., COLAS, J.-F., BOLAÑOS-JIMENEZ, F., TOURNOIS, C., LAUNAY, J.-M., MAROTEAUX, L.
• Format: Article
• Language: English
• Subjects: Animals; Cell Differentiation; Embryonic and Fetal Development; Gene Expression Regulation, Developmental; Genes, ras; Life Sciences; Mice; Neurons and Cognition; Proto-Oncogene Proteins p21(ras) - genetics; Receptor, Serotonin, 5-HT2B; Receptors, Serotonin - genetics; Receptors, Serotonin - physiology; Serotonin - physiology
• ISSN: 0077-8923; 1749-6632
• DOI: 10.1111/j.1749-6632.1998.tb10174.x

5-HT2B receptors, in addition to phospholipase C stimulation, are able to trigger activation of the proto-oncogene product p21ras. During mouse embryogenesis, a peak of 5-HT2B receptor expression is detected at the neurulation stage; we localized the 5-HT2B expression in neural crest cells, heart myocardium, and somites. The requirement for functional 5-HT2B receptors shortly after gastrulation, is supported by culture of embryos exposed to 5-HT2B-high affinity antagonist such as ritanserin, which induces morphological defects in the cephalic region, heart and neural tube. Functional 5-HT2B receptors are also expressed during the serotonergic differentiation of the mouse F9 teratocarcinoma-derived clonal cell line 1C11. Upon 2 days of induction by cAMP, 5-HT2B receptors become functional, and on day 4, the appearance of 5-HT2A receptors coincides with the onset of active serotonin transporter by these cells. Active serotonin uptake is modulated by serotonin suggesting autoreceptor functions for 5-HT2B receptors.