Transposable elements behavior following viral genomic stress in Drosophila melanogaster inbred line

• Published in: Journal of molecular evolution 1996-07, Vol.43 (1), p.19-27
• Main Authors: Jouan-Dufournel, I, Cosset, F L, Contamine, D, Verdier, G, Biémont, C
• Format: Article
• Language: English
• Subjects: AIDS/HIV; Animals; Avian Leukosis Virus - genetics; Base Sequence; Biochemistry; Biochemistry, Molecular Biology; Chromosomes; DNA Primers; DNA Transposable Elements; Drosophila melanogaster; Drosophila melanogaster - embryology; Drosophila melanogaster - genetics; Embryo, Nonmammalian; Gene Deletion; Genes, env; Genome; In Situ Hybridization; Inbreeding; Life Sciences; Molecular Sequence Data; Plasmids; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Transfection; Virion
• ISSN: 0022-2844; 1432-1432
• DOI: 10.1007/BF02352295

To analyze the behavior of endogenous transposable elements under genomic stress, a Drosophila melanogaster inbred line was submitted to three kinds of viral perturbations. First, a retroviral plasmid containing the avian Rous Associated Virus type 2 (RAV-2) previously deleted for the viral envelope coding gene (env) was introduced by P element transformation into the Drosophila genome. An insertion of this avian retroviral sequence was detected by in situ hybridization in site 53C on polytene chromosome arm 2R. Second, Drosophila embryos were injected with RAV-2 particles produced by cell culture after transfection with the retroviral plasmid. Third, the Drosophila melanogaster inbred line was stably infected by the sigma native virus. It appears that neither the offspring of the flies in which the viral DNA was found integrated nor those from the infected sigma flies showed copia or mdg1 element mobilization. Injection of the avian RAV-2 particles led, however, to the observation of somatic transpositions of mdg1 element on the 2L chromosome, the copia element insertion pattern remaining stable. Thus, endogenous transposable elements show more instability in sublines injected with exogenous viral particles than in a transgenic subline containing a foreign viral insert, all transposable elements not being equally sensitive to such genomic stress.