Monitoring complex bacterial communities using culture-independent molecular techniques: application to soil environment

Over the last decade, important advances in molecular biology led to the development of culture-independent approaches to describing bacterial communities. These new strategies, based on the analysis of DNA directly extracted from environmental samples, circumvent the steps of isolation and culturin...

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Bibliographic Details
Published in:Research in Microbiology 2000-04, Vol.151 (3), p.167-177
Main Authors: Ranjard, Lionel, Poly, Franck, Nazaret, Sylvie
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Animal, plant and microbial ecology
Bacteria - genetics
Bacteria - growth & development
bacterial community
Biochemistry and biology
Biological and medical sciences
Chemical, physicochemical, biochemical and biological properties
DNA
DNA, Bacterial - analysis
Ecosystem
Fundamental and applied biological sciences. Psychology
genetic diversity
genetic structure
Life Sciences
Microbial ecology
Microbiology
Microbiology and Parasitology
molecular biology
Molecular Biology - methods
Physics, chemistry, biochemistry and biology of agricultural and forest soils
Soil
Soil Microbiology
Soil science
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Summary:Over the last decade, important advances in molecular biology led to the development of culture-independent approaches to describing bacterial communities. These new strategies, based on the analysis of DNA directly extracted from environmental samples, circumvent the steps of isolation and culturing of bacteria, which are known for their selectivity leading to a non-representative view of the extent of bacterial diversity. This review provides an overview of the potentials and limitations of some molecular approaches currently used in microbial ecology. Examples of applications to the study of indigenous soil microbial community illustrate the feasibility and the power of such approaches.
ISSN:0923-2508
1769-7123
0923-2508
DOI:10.1016/S0923-2508(00)00136-4