Acetogenesis from H2 and CO2 by methane- and non-methane-producing human colonic bacterial communities

Abstract The purpose of the study was to define the potential for reductive acetogenesis of colonic microflora from six non\u2010methane\u2010 and four methane\u2010excreting human subjects in relation to numbers of the different H2\u2010utilizing microorganisms. Faecal bacterial suspensions were in...

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Published in:FEMS microbiology ecology 1996-03, Vol.19 (3), p.193-202
Main Authors: Bernalier, Annick, Lelait, Marielle, Rochet, Violaine, Grivet, Jean-Philippe, Gibson, Glenn R., Durand, Michelle
Format: Article
Language:English
Subjects:
13C‐NMR
Acetic acid
Acetogenesis
Animal, plant and microbial ecology
Biological and medical sciences
Carbon dioxide
Ecology
Fundamental and applied biological sciences. Psychology
Human faeces
Hydrogen
Interrelationships
Life Sciences
Methane
Methanogenesis
Methanogenic bacteria
Microbial ecology
Microbiology
Microbiology and Parasitology
Microflora
Microorganisms
NMR
Normal microflora of man and animals. Rumen
Nuclear magnetic resonance
Reductive acetogenesis
Sulfates
Vapor phases
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Summary:Abstract The purpose of the study was to define the potential for reductive acetogenesis of colonic microflora from six non\u2010methane\u2010 and four methane\u2010excreting human subjects in relation to numbers of the different H2\u2010utilizing microorganisms. Faecal bacterial suspensions were incubated in the presence of NaH13CO3 and under a gas phase composed of either 100% N2 (control) or 80% H2\u201320% N2. The effects of a specific methanogenesis inhibitor or of sulfate supplementation were also determined. Quantitative nuclear magnetic resonance showed the presence of both single\u2010 and double\u2010labelled acetate in all incubations under hydrogen. H2/CO2\u2010acetogenesis appears to be a quantitatively important activity only in the presence of very low numbers of methanogens. Inhibition of methanogenesis induced a large increase in 13CO2 incorporation into acetate in CH4\u2010producing samples. These results showed that methanogens can efficiently outcompete acetogens in human colonic contents. In contrast, no clear\u2010cut competition for H2 between acetogenesis and dissimilatory sulfate\u2010reduction could be demonstrated. A slight reduction of the acetogenic activity was only observed at the highest sulfate addition (100 mM).
ISSN:0168-6496
1574-6941
DOI:10.1111/j.1574-6941.1996.tb00212.x