TolB protein of Escherichia coli K‐12 interacts with the outer membrane peptidoglycan‐associated proteins Pal, Lpp and OmpA

The Tol–Pal proteins of Escherichia coli are involved in maintaining outer membrane integrity. Transmembrane domains of TolQ, TolR and TolA interact in the cytoplasmic membrane, while TolB and Pal form a complex near the outer membrane. TolB and the central domain of TolA interact in vitro with the...

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Bibliographic Details
Published in:Molecular microbiology 1998-07, Vol.29 (1), p.359-367
Main Authors: Clavel, Thierry, Germon, Pierre, Vianney, Anne, Portalier, Raymond, Lazzaroni, Jean Claude
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Outer Membrane Proteins - biosynthesis
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - metabolism
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Carrier Proteins - metabolism
Cross-Linking Reagents
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli Proteins
Life Sciences
Lipoproteins - genetics
Lipoproteins - metabolism
Membrane Proteins - metabolism
Microbiology and Parasitology
Molecular Sequence Data
Mutagenesis
Peptidoglycan - genetics
Peptidoglycan - metabolism
Periplasmic Proteins
Proteoglycans
Sequence Homology, Amino Acid
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Summary:The Tol–Pal proteins of Escherichia coli are involved in maintaining outer membrane integrity. Transmembrane domains of TolQ, TolR and TolA interact in the cytoplasmic membrane, while TolB and Pal form a complex near the outer membrane. TolB and the central domain of TolA interact in vitro with the outer membrane porins. In this study, both genetic and biochemical analyses were carried out to analyse the links between TolB, Pal and other components of the cell envelope. It was shown that TolB could be cross‐linked in vivo with Pal, OmpA and Lpp, while Pal was associated with TolB and OmpA. The isolation of pal and tolB mutants disrupting some interactions between these proteins represents a first approach to characterizing the residues contributing to the interactions. We propose that TolB and Pal are part of a multiprotein complex that links the peptidoglycan to the outer membrane. The Tol–Pal proteins might form transenvelope complexes that bring the two membranes into close proximity and help some outer membrane components to reach their final destination.
ISSN:0950-382X
1365-2958
DOI:10.1046/j.1365-2958.1998.00945.x