Short-chain fatty acids induce cytoskeletal and extracellular protein modifications associated with modulation of proliferation on primary culture of rat intestinal smooth muscle cells

Short-chain fatty acids are the main end products of bacterial fermentation of carbohydrates. Their role on the metabolism and biology of colonocytes is now well characterized. However, the functional consequences of their presence on intestinal smooth muscle cells remain poorly studied. We aimed to...

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Bibliographic Details
Published in:Digestive diseases and sciences 2000-08, Vol.45 (8), p.1623-1630
Main Authors: LE BLAY, Gwenaëlle, BLOTTIERE, Hervé M, FERRIER, Laurent, LE FOLL, Eric, BONNET, Christian, GALMICHE, Jean-Paul, CHERBUT, Christine
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Butyrates - pharmacology
Cell Division - drug effects
Cells, Cultured
Colon - cytology
Cytoskeletal Proteins - biosynthesis
Digestive system
Extracellular Matrix Proteins - biosynthesis
Fatty Acids, Volatile - pharmacology
Ileum - cytology
Intestines - cytology
Investigative techniques, diagnostic techniques (general aspects)
Life Sciences
Male
Medical sciences
Muscle, Smooth - cytology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Rats
Rats, Wistar
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Summary:Short-chain fatty acids are the main end products of bacterial fermentation of carbohydrates. Their role on the metabolism and biology of colonocytes is now well characterized. However, the functional consequences of their presence on intestinal smooth muscle cells remain poorly studied. We aimed to assess the effect of different short-chain fatty acids on ileal and colonic smooth muscle cells in primary culture and on A7R5 line. Butyrate (above 0.1 mM) inhibited A7R5 cell proliferation, while at low concentration (0.05 to 0.5 mM) butyrate significantly stimulated the proliferation of ileal and colonic myocytes in primary culture. An inhibition was observed at higher concentrations. Collagenous and noncollagenous protein synthesis was stimulated by butyrate. Moreover, butyrate stimulated actin and myosin expression. Thus, butyrate, which is produced by dietary fiber fermentation, may affect intestinal muscles by directly acting at the molecular level on myocytes.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1005529414765