xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria

We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encode...

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Bibliographic Details
Published in:FEMS microbiology letters 1999-12, Vol.181 (1), p.145-152
Main Authors: Devillard, E, Newbold, C.J, Scott, K.P, Forano, E, Wallace, R.J, Jouany, J.P, Flint, H.J
Format: Article
Language:English
Subjects:
active sites
Amino Acid Sequence
Anaerobiosis
Animals
Bacteria
Base Sequence
Biochemistry. Physiology. Immunology. Molecular biology
Biodegradation
Biological and medical sciences
cDNA libraries
Cell walls
Ciliophora - enzymology
Ciliophora - genetics
Cloning
complementary DNA
DNA, Complementary - genetics
Domains
Electrophoresis, Polyacrylamide Gel
Enzymes
Eukaryotes
eukaryotic cells
Fundamental and applied biological sciences. Psychology
Fungi
Glucans - metabolism
Glycoside hydrolase
glycosides
Gram-positive bacteria
Gram-Positive Bacteria - enzymology
Gram-Positive Bacteria - genetics
Hydrolase
Immunoblotting
Life Sciences
Microbiology
Microbiology and Parasitology
Molecular Sequence Data
Phylogeny
Polyplastron multivesiculatum
Polysaccharides
Protozoa
Rumen
Rumen - parasitology
Sequence Analysis, DNA
sequence homology
Xylan Endo-1,3-beta-Xylosidase
Xylanase
xylanases
Xylans - metabolism
Xylosidases - chemistry
Xylosidases - genetics
Xylosidases - metabolism
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Summary:We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb08837.x