Partial Sequencing of the hrpB and Endoglucanase Genes Confirms and Expands the Known Diversity within the Ralstonia solanacearum Species Complex

We determined partial hrpB and endoglucanase genes sequences for 30 strains of Ralstonia solanacearum and one strain of the blood disease bacterium (BDB), a close relative of Ralstonia solanacearum. Sequence comparisons showed high levels of variability within these two regions of the genome involve...

Full description

Saved in:
Bibliographic Details
Published in:Systematic and applied microbiology 2000-12, Vol.23 (4), p.479-486
Main Authors: Poussier, Stephane, Prior, Philippe, Luisetti, Jacques, Hayward, Chris, Fegan, Mark
Format: Article
Language:English
Subjects:
Africa
African strains
Bacterial Proteins - analysis
Bacterial Proteins - classification
Bacterial Proteins - genetics
bacterial wilt
Bacteriology
Betaproteobacteria - classification
Betaproteobacteria - genetics
Betaproteobacteria - isolation & purification
Biological and medical sciences
Cellulase - analysis
Cellulase - classification
Cellulase - genetics
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
DNA-Binding Proteins
endoglucanase
endoglucanase gene sequences
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
genetic diversity
Genetic Variation
Genetics
hrpB gene
hrpB gene sequences
Life Sciences
Microbiology
Microbiology and Parasitology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phylogeny
Phytopathology and phytopharmacy
Ralstonia solanacearum
Repressor Proteins - analysis
Repressor Proteins - classification
Repressor Proteins - genetics
RNA, Ribosomal, 16S - analysis
Species Specificity
Transcription Factors
Vegetal Biology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We determined partial hrpB and endoglucanase genes sequences for 30 strains of Ralstonia solanacearum and one strain of the blood disease bacterium (BDB), a close relative of Ralstonia solanacearum. Sequence comparisons showed high levels of variability within these two regions of the genome involved in pathogenicity. Phylogenetic analysis based upon sequence comparisons of these two regions revealed three major clusters comprising all Ralstonia solanacearum isolates, the BDB strain constituted a phylogenetically distinct entity. Cluster 1 and cluster 2 corresponded to the previously defined divisions 1 and 2 of Ralstonia solanacearum. Moreover, two subclusters could be identified within cluster 2. The last cluster, designated cluster 3 in this study, included biovar 1 and N2 strains originating from Africa. This recently described group of strains was confirmed to be clearly different from the other strains suggesting a separate evolution from those of both divisions 1 and 2.
ISSN:0723-2020
1618-0984
DOI:10.1016/S0723-2020(00)80021-1