Use of Agrobacterium rhizogenes to create transgenic apple trees having an altered organogenic response to hormones

The apple rootstock, M26, was genetically and phenotypically transformed using the Agrobacterium wild-type strain, A4. First, chimeric plants were obtained having transformed roots and normal aerial parts. Transformed plants were then produced through regeneration from transformed roots. Transformat...

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Bibliographic Details
Published in:Theoretical and applied genetics 1992-10, Vol.85 (1), p.105-109
Main Authors: Lambert, C. (Angers Univ. (France). Faculte des Sciences. Lab. de Biologie Vegetale), Tepfer, D
Format: Article
Language:English
Subjects:
AGROBACTERIUM RHIZOGENES
Apple
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
CRECIMIENTO
CROISSANCE
Fundamental and applied biological sciences. Psychology
GENETIC TRANSFORMATION
Genetics
Genetics of eukaryotes. Biological and molecular evolution
GROWTH
Life Sciences
Malus
MALUS SYLVESTRIS
PLANT GROWTH SUBSTANCES
Pteridophyta, spermatophyta
RACINE
RAICES
Root development
ROOTS
SUBSTANCE DE CROISSANCE VEGETALE
SUSTANCIAS DE CRECIMIENTO VEGETAL
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Vegetals
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Summary:The apple rootstock, M26, was genetically and phenotypically transformed using the Agrobacterium wild-type strain, A4. First, chimeric plants were obtained having transformed roots and normal aerial parts. Transformed plants were then produced through regeneration from transformed roots. Transformation was demonstrated by molecular hybridization and opine analysis. The effects of hormones on organogenesis was altered in transformants: cytokinins were required to form roots, whereas auxin was toxic at the concentration used to induce rooting in the control.
ISSN:0040-5752
1432-2242
DOI:10.1007/bf00223851