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Influence of the degree of polymerization of oligogalacturonates and of esterification pattern of pectin on their recognition by monoclonal antibodies

The ability of galacturonic and oligogalacturonic acids with degrees of polymerization (DP) from 2 to 10 to inhibit the recognition of homopolygalacturonic acid by a monoclonal antibody specific for dimers of pectin (F Liners, J-J Letesson, C Didembourg, P Van Cutsem [1989] Plant Physiol 91: 1419-14...

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Published in:	Plant physiology (Bethesda) 1992-07, Vol.99 (3), p.1099-1104
Main Authors:	Liners, F. (Universitaires Notre-Dame de la Paix, Namur, Belgium), Thibault, J.F, Cutsem, P. van
Format:	Article
Language:	English
Subjects:	Antibodies ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES AZUCARES ACIDOS Biological and medical sciences Calcium Cell biochemistry Cell physiology Cellular and Structural Biology Centrifugation Dimers Enzyme linked immunosorbent assay Epitopes ESTERIFICACION ESTERIFICATION FISIOLOGIA VEGETAL Fundamental and applied biological sciences. Psychology Genetics Life Sciences Magnesium Monoclonal antibodies MORFOGENESIS MORPHOGENESE Oligomers PECTINAS PECTINE PHYSIOLOGIE VEGETALE Plant physiology and development Plants Plants genetics POLIMERIZACION POLYMERISATION SUCRE ACIDE
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creator	Liners, F. (Universitaires Notre-Dame de la Paix, Namur, Belgium) Thibault, J.F Cutsem, P. van
description	The ability of galacturonic and oligogalacturonic acids with degrees of polymerization (DP) from 2 to 10 to inhibit the recognition of homopolygalacturonic acid by a monoclonal antibody specific for dimers of pectin (F Liners, J-J Letesson, C Didembourg, P Van Cutsem [1989] Plant Physiol 91: 1419-1424) has been tested by enzyme-linked immunosorbent assays. Oligomers of DP9 and above preincubated with the antibodies clearly inhibited the association between the antibodies and immobilized pectin. A minimum DP of nine consecutive galacturonic residues is thus necessary to be associated through calcium cations to form dimers. Randomly deesterified pectin was recognized by the antibody if its degree of methylesterification was 30%, whereas blockwise deesterified pectin was recognized up to 40% of methylesterification. The replacement of calcium ions by magnesium prevented the recognition of polygalacturonic acid by the antibody
doi_str_mv	10.1104/pp.99.3.1099
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Randomly deesterified pectin was recognized by the antibody if its degree of methylesterification was 30%, whereas blockwise deesterified pectin was recognized up to 40% of methylesterification. 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Psychology ; Genetics ; Life Sciences ; Magnesium ; Monoclonal antibodies ; MORFOGENESIS ; MORPHOGENESE ; Oligomers ; PECTINAS ; PECTINE ; PHYSIOLOGIE VEGETALE ; Plant physiology and development ; Plants ; Plants genetics ; POLIMERIZACION ; POLYMERISATION ; SUCRE ACIDE</subject><ispartof>Plant physiology (Bethesda), 1992-07, Vol.99 (3), p.1099-1104</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><rights>1993 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-66f7ac692db8c1e9d1d4220e5beaeafa40e1f1ebac865f88cf8dee3c17ea5f93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4274473$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4274473$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4551540$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16668976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02704226$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Liners, F. (Universitaires Notre-Dame de la Paix, Namur, Belgium)</creatorcontrib><creatorcontrib>Thibault, J.F</creatorcontrib><creatorcontrib>Cutsem, P. van</creatorcontrib><title>Influence of the degree of polymerization of oligogalacturonates and of esterification pattern of pectin on their recognition by monoclonal antibodies</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The ability of galacturonic and oligogalacturonic acids with degrees of polymerization (DP) from 2 to 10 to inhibit the recognition of homopolygalacturonic acid by a monoclonal antibody specific for dimers of pectin (F Liners, J-J Letesson, C Didembourg, P Van Cutsem [1989] Plant Physiol 91: 1419-1424) has been tested by enzyme-linked immunosorbent assays. Oligomers of DP9 and above preincubated with the antibodies clearly inhibited the association between the antibodies and immobilized pectin. A minimum DP of nine consecutive galacturonic residues is thus necessary to be associated through calcium cations to form dimers. Randomly deesterified pectin was recognized by the antibody if its degree of methylesterification was 30%, whereas blockwise deesterified pectin was recognized up to 40% of methylesterification. The replacement of calcium ions by magnesium prevented the recognition of polygalacturonic acid by the antibody</description><subject>Antibodies</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>AZUCARES ACIDOS</subject><subject>Biological and medical sciences</subject><subject>Calcium</subject><subject>Cell biochemistry</subject><subject>Cell physiology</subject><subject>Cellular and Structural Biology</subject><subject>Centrifugation</subject><subject>Dimers</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Epitopes</subject><subject>ESTERIFICACION</subject><subject>ESTERIFICATION</subject><subject>FISIOLOGIA VEGETAL</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Life Sciences</subject><subject>Magnesium</subject><subject>Monoclonal antibodies</subject><subject>MORFOGENESIS</subject><subject>MORPHOGENESE</subject><subject>Oligomers</subject><subject>PECTINAS</subject><subject>PECTINE</subject><subject>PHYSIOLOGIE VEGETALE</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Plants genetics</subject><subject>POLIMERIZACION</subject><subject>POLYMERISATION</subject><subject>SUCRE ACIDE</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNpFkc-L1DAUx4Mo7jh68yQiPQgiOGPSpD9yXBZ1FwY8uJ7Da_rSzZI2NWkXxj_Ev9d0Ouye8t77fvIh4RHyltE9Y1R8Hce9lHu-Z1TKZ2TDCp7v8kLUz8mG0lTTupYX5FWM95RSxpl4SS5YWZa1rMoN-XczGDfjoDHzJpvuMGuxC3jqRu-OPQb7Fybrh2Xine18Bw70NAc_wIQxg6FdIoxTQo3VKzzClPrTpRH1ZFM1LHobsoDad4M9Yc0x6_3gtUsyl1STbXxrMb4mLwy4iG_O55bcfv92e3W9O_z8cXN1edhpLqtpV5amAl3KvG1qzVC2rBV5TrFoEBAMCIrMMGxA12Vh6lqbukXkmlUIhZF8Sz6v2jtwagy2h3BUHqy6vjyoZUbziiZj-cAS-2llx-D_zOm7qrdRo3MwoJ-jqjgXUoiySOSXldTBxxjQPKoZVcvO1DgqKRVXy84S_uEsnpse2yf4vKQEfDwDEDU4E2DQNj5yoihYIWjC3q_YfZx8eIrzSoj0uC15t8YGvIIuJMPvX5JzJmXN_wNo97UU</recordid><startdate>19920701</startdate><enddate>19920701</enddate><creator>Liners, F. 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(Universitaires Notre-Dame de la Paix, Namur, Belgium) ; Thibault, J.F ; Cutsem, P. van</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-66f7ac692db8c1e9d1d4220e5beaeafa40e1f1ebac865f88cf8dee3c17ea5f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Antibodies</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>AZUCARES ACIDOS</topic><topic>Biological and medical sciences</topic><topic>Calcium</topic><topic>Cell biochemistry</topic><topic>Cell physiology</topic><topic>Cellular and Structural Biology</topic><topic>Centrifugation</topic><topic>Dimers</topic><topic>Enzyme linked immunosorbent assay</topic><topic>Epitopes</topic><topic>ESTERIFICACION</topic><topic>ESTERIFICATION</topic><topic>FISIOLOGIA VEGETAL</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Life Sciences</topic><topic>Magnesium</topic><topic>Monoclonal antibodies</topic><topic>MORFOGENESIS</topic><topic>MORPHOGENESE</topic><topic>Oligomers</topic><topic>PECTINAS</topic><topic>PECTINE</topic><topic>PHYSIOLOGIE VEGETALE</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Plants genetics</topic><topic>POLIMERIZACION</topic><topic>POLYMERISATION</topic><topic>SUCRE ACIDE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liners, F. (Universitaires Notre-Dame de la Paix, Namur, Belgium)</creatorcontrib><creatorcontrib>Thibault, J.F</creatorcontrib><creatorcontrib>Cutsem, P. van</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liners, F. (Universitaires Notre-Dame de la Paix, Namur, Belgium)</au><au>Thibault, J.F</au><au>Cutsem, P. van</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of the degree of polymerization of oligogalacturonates and of esterification pattern of pectin on their recognition by monoclonal antibodies</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1992-07-01</date><risdate>1992</risdate><volume>99</volume><issue>3</issue><spage>1099</spage><epage>1104</epage><pages>1099-1104</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The ability of galacturonic and oligogalacturonic acids with degrees of polymerization (DP) from 2 to 10 to inhibit the recognition of homopolygalacturonic acid by a monoclonal antibody specific for dimers of pectin (F Liners, J-J Letesson, C Didembourg, P Van Cutsem [1989] Plant Physiol 91: 1419-1424) has been tested by enzyme-linked immunosorbent assays. Oligomers of DP9 and above preincubated with the antibodies clearly inhibited the association between the antibodies and immobilized pectin. A minimum DP of nine consecutive galacturonic residues is thus necessary to be associated through calcium cations to form dimers. Randomly deesterified pectin was recognized by the antibody if its degree of methylesterification was 30%, whereas blockwise deesterified pectin was recognized up to 40% of methylesterification. The replacement of calcium ions by magnesium prevented the recognition of polygalacturonic acid by the antibody</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16668976</pmid><doi>10.1104/pp.99.3.1099</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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subjects	Antibodies ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES AZUCARES ACIDOS Biological and medical sciences Calcium Cell biochemistry Cell physiology Cellular and Structural Biology Centrifugation Dimers Enzyme linked immunosorbent assay Epitopes ESTERIFICACION ESTERIFICATION FISIOLOGIA VEGETAL Fundamental and applied biological sciences. Psychology Genetics Life Sciences Magnesium Monoclonal antibodies MORFOGENESIS MORPHOGENESE Oligomers PECTINAS PECTINE PHYSIOLOGIE VEGETALE Plant physiology and development Plants Plants genetics POLIMERIZACION POLYMERISATION SUCRE ACIDE
title	Influence of the degree of polymerization of oligogalacturonates and of esterification pattern of pectin on their recognition by monoclonal antibodies
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