Specific serological detection of the transmissible virus in pea seed infected by pea seed-borne mosaic virus

The capsid protein of pea seed-borne mosaic virus (PSbMV), analyzed in mature pea seed, had a molecular mass of 33 kDa in infected embryos but a molecular mass of only 29-27 kDa in seed testas. A proteinase activity, induced in pea seed testas, efficiently cleaved the capsid protein in vitro as did...

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Bibliographic Details
Published in:Phytopathology 1994, Vol.84 (7), p.756-760
Main Authors: Masmoudi, K. (Institut National de la Recherche Agronomique, Versailles, France), Suhas, M, Khetarpal, R.K, Maury, Y
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DIAGNOSTIC
DIAGNOSTICO
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
GRAINE
IMMUNOLOGIE
INMUNOLOGIA
Life Sciences
PATHOGENESE
PATOGENESIS
Phytopathology and phytopharmacy
Phytopathology. Animal pests. Plant and forest protection
PISUM SATIVUM
Plant viruses and viroids
PODER PATOGENO
POTYVIRUS
POUVOIR PATHOGENE
PROTEINAS
PROTEINE
SEMILLA
TRANSMISION DE ENFERMEDADES
TRANSMISSION DES MALADIES
Vegetal Biology
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
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Description
Summary:The capsid protein of pea seed-borne mosaic virus (PSbMV), analyzed in mature pea seed, had a molecular mass of 33 kDa in infected embryos but a molecular mass of only 29-27 kDa in seed testas. A proteinase activity, induced in pea seed testas, efficiently cleaved the capsid protein in vitro as did a mild trypsin treatment. The occurrence of this proteinase activity was independent of the infection; its physiological induction in testas occurred at a development stage that corresponds to the active growth of the embryo. The use of an antiserum specific to the deleted part of the protein enabled detection of PSbMV only in embryos. This serological differentiation between the seed-transmitted and seedborne virus may have a direct application in the routine screening of pea seed lots
ISSN:0031-949X
1943-7684
0031-949X
DOI:10.1094/Phyto-84-756