Specific serological detection of the transmissible virus in pea seed infected by pea seed-borne mosaic virus

• Published in: Phytopathology 1994, Vol.84 (7), p.756-760
• Main Authors: Masmoudi, K. (Institut National de la Recherche Agronomique, Versailles, France), Suhas, M, Khetarpal, R.K, Maury, Y
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DIAGNOSTIC; DIAGNOSTICO; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control; GRAINE; IMMUNOLOGIE; INMUNOLOGIA; Life Sciences; PATHOGENESE; PATOGENESIS; Phytopathology and phytopharmacy; Phytopathology. Animal pests. Plant and forest protection; PISUM SATIVUM; Plant viruses and viroids; PODER PATOGENO; POTYVIRUS; POUVOIR PATHOGENE; PROTEINAS; PROTEINE; SEMILLA; TRANSMISION DE ENFERMEDADES; TRANSMISSION DES MALADIES; Vegetal Biology; VIRUS DE LAS PLANTAS; VIRUS DES VEGETAUX
• ISSN: 0031-949X; 1943-7684; 0031-949X
• DOI: 10.1094/Phyto-84-756

The capsid protein of pea seed-borne mosaic virus (PSbMV), analyzed in mature pea seed, had a molecular mass of 33 kDa in infected embryos but a molecular mass of only 29-27 kDa in seed testas. A proteinase activity, induced in pea seed testas, efficiently cleaved the capsid protein in vitro as did a mild trypsin treatment. The occurrence of this proteinase activity was independent of the infection; its physiological induction in testas occurred at a development stage that corresponds to the active growth of the embryo. The use of an antiserum specific to the deleted part of the protein enabled detection of PSbMV only in embryos. This serological differentiation between the seed-transmitted and seedborne virus may have a direct application in the routine screening of pea seed lots