Production and characterization of monoclonal antibodies directed against Eimeria falciformis and cross-reactive with sporozoites from two species of avian coccidia

Monoclonal antibodies (mAbs) were obtained against the surface antigens of the Eimeria falciformis sporozoite by immunizing mice with whole homogenized sporozoite. The hybridomas were selected by their reactivities against oocyst extracts, then against glutaraldehyde-treated sporozoites. Three mAbs...

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Bibliographic Details
Published in:Parasitology research (1987) 1994, Vol.80 (5), p.396-402
Main Authors: KAZANJI, M, ABDELKEBIR RHALEM, PERY, P
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibodies, Protozoan - immunology
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Birds - parasitology
Blotting, Western
Coccidia - cytology
Coccidia - growth & development
Coccidia - immunology
Cross Reactions
Eimeria - immunology
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Invertebrates
Life Sciences
Membrane Proteins - immunology
Mice
Mice, Inbred BALB C
Microbiology and Parasitology
Protozoa
Protozoan Proteins - immunology
Species Specificity
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Summary:Monoclonal antibodies (mAbs) were obtained against the surface antigens of the Eimeria falciformis sporozoite by immunizing mice with whole homogenized sporozoite. The hybridomas were selected by their reactivities against oocyst extracts, then against glutaraldehyde-treated sporozoites. Three mAbs recognized both the surface of E. falciformis, E. tenella, and E. acervulina and their refractile bodies, whereas a fourth mAb recognized only one epitope on the refractile bodies. All mAbs bound to the same immunoaffinity-purified antigens in Western-blot analysis (P27 for E. falciformis and P25 for E. tenella and E. acervulina). Thus, the mAbs define at least two shared epitopes between sporozoite antigens from different eimerian species. Two of these mAbs are involved in the in vitro phagocytosis of E. falciformis sporozoites by macrophages and also in their lysis by neutrophils. Altogether, these properties showed that the four mAbs came from different activated B-cells. The P27 antigen recognized by our mAbs represents a major target of the in vitro destructive immune response.
ISSN:0932-0113
1432-1955
DOI:10.1007/BF00932377