In vitro processing of the RNA-2-encoded polyprotein of two nepoviruses: tomato black ring virus and grapevine chrome mosaic virus

• Published in: Journal of general virology 1991-02, Vol.72 (2), p.247-252
• Main Authors: Demangeat, Gerard, Hemmer, Odile, Fritsch, Christiane, Gall, Olivier Le, Candresse, Thierry
• Format: Article
• Language: English
• Subjects: Animals; Electrophoresis, Polyacrylamide Gel; grapevine chrome mosaic virus; Life Sciences; Microbiology and Parasitology; Mosaic Viruses - genetics; Mosaic Viruses - metabolism; Plant Viruses - genetics; Plant Viruses - metabolism; Protein Biosynthesis; Protein Processing, Post-Translational; Proteins - genetics; Proteins - metabolism; proteolysis; Rabbits; reticulocytes; RNA, Viral - genetics; Viral Proteins - genetics; Viral Proteins - metabolism; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-72-2-247

1 Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg Cédex and 2 Station de Pathologie Végétale de l'INRA, Centre de Recherches de Bordeaux, 33883 Villenave d'Ornon Cédex, France In vitro translation of RNA-2 of each of two closely related nepoviruses, tomato black ring virus (TBRV) and grapevine chrome mosaic virus (GCMV), in a rabbit reticulocyte lysate resulted in the synthesis of single polypeptides of 150K and 146K respectively. Processing of these polyproteins occurred after the addition of translation products of homologous RNA-1. The positions of the cleavage products within the polyproteins were determined. From the N to the C terminus, M r values for the proteins were 50K, 46K and 59K for TBRV and 44K, 46K and 56K for GCMV. TBRV RNA-1 translation products also cleaved the polyproteins encoded by GCMV RNA-2 which suggests that the cleavage sites in the two polyproteins are similar. Received 17 July 1990; accepted 12 October 1990.