Embryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture

Isolated microspores of various populations of three varieties of the Chinese cabbage pakchoi (Brassica rapa ssp. chinensis) were cultivated in vitro on NLN82 medium (Lichter 1982) and embryos and plantlets obtained with nine cultivars. The best embryo yield per bud was 57.4. A 33°C one day heat tre...

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Bibliographic Details
Published in:Plant cell reports 1994-05, Vol.13 (8), p.447-450
Main Authors: MING QING CAO, YAN LI, FAN LIU, DORE, C
Format: Article
Language:English
Subjects:
Anther and ovule culture, utilization for haploid production
Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Genetics
Life Sciences
Methods. Procedures. Technologies
Plant cells and fungal cells
Plants genetics
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Summary:Isolated microspores of various populations of three varieties of the Chinese cabbage pakchoi (Brassica rapa ssp. chinensis) were cultivated in vitro on NLN82 medium (Lichter 1982) and embryos and plantlets obtained with nine cultivars. The best embryo yield per bud was 57.4. A 33°C one day heat treatment was generally necessary to induce embryogenesis. Analysis of ploidy level through flow cytometry for two cultivars indicated that haploids were present.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00231964