Quantitative three-dimensional study on the position of the female gametophyte and its constituent cells as a prerequisite for corn (Zea mays) transformation

The position of the embryo sac in the spikelet and of the embryo sac's constituent cells within the sporophytic tissues of Zea mays was localized by scanning electron microscopy, serial thick sectioning, and computer three-dimensional reconstruction. Within certain limits, the embryo sac is con...

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Bibliographic Details
Published in:Theoretical and applied genetics 1990, Vol.79 (1), p.72-76
Main Authors: Wagner, V.T, Dumas, C, Mogensen, H.L
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
embryo (plant)
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
genetic transformation
Genetics
laboratory techniques
Life Sciences
Methods. Procedures. Technologies
microinjection
plant anatomy
plant morphology
ultrastructure
Vectors (cloning, transfer, expression). Insertion sequences and transposons
Zea mays
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Summary:The position of the embryo sac in the spikelet and of the embryo sac's constituent cells within the sporophytic tissues of Zea mays was localized by scanning electron microscopy, serial thick sectioning, and computer three-dimensional reconstruction. Within certain limits, the embryo sac is consistently oriented in the same position inside of the spikelet. This information is a prerequisite for successful microinjections into the in situ female cells of Zea mays.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00223789