Promoter and transcription start site of human and rabbit butyrylcholinesterase genes

Two kilobase segments of the 5'-untranslated regions of the human and rabbit butyrylcholinesterase (BCHE) genes were characterized. The sequences shared extensive identity except for a 333-base pair (bp) Alu repeat present only in human BCHE. One single transcription start site was found in bot...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1994-08, Vol.269 (33), p.20829-20837
Main Authors: JBILO, O, TOUTANT, J.-P, VATSIS, K. P, CHATONNET, A, LOCKRIDGE, O
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Base Sequence
Biological and medical sciences
Blotting, Northern
Butyrylcholinesterase - genetics
Chloramphenicol O-Acetyltransferase - genetics
Computer Science
DNA - chemistry
DNA - genetics
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Hydrolases
Life Sciences
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Conformation
Promoter Regions, Genetic
Rabbits
RNA, Messenger - metabolism
Sequence Alignment
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two kilobase segments of the 5'-untranslated regions of the human and rabbit butyrylcholinesterase (BCHE) genes were characterized. The sequences shared extensive identity except for a 333-base pair (bp) Alu repeat present only in human BCHE. One single transcription start site was found in both genes with the techniques of primer extension, amplification of the 5'-end of mRNA, and RNase protection. Cap sites in human and rabbit BCHE genes were found in strictly homologous positions. In human BCHE, the transcription start site was found 157 bp upstream of Met-28, the translation start site. Potential regulatory elements in both promoters included one AP1 site and multiple sites for topoisomerase, Oct-1 and PEA-3. Transient expression of BCHE-reporter gene constructs showed that a 194-bp fragment of the 5'-flanking region of human BCHE and a 570-bp fragment of rabbit BCHE were sufficient for promoting chloramphenicol acetyltransferase activity in HeLa cells. No consensus TATA and CAAT boxes were found. However, the sequence around the transcription start site exhibited homology with initiator elements found in other TATA-less promoters in developmentally regulated genes.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)31897-5