Cell wall polysaccharide interactions in maize bran

Sequential extractions with alkali have been carried out in order to study the nature of linkages which hold heteroxylans in maize bran cell walls. Treatment with 0.5 m sodium hydroxide at 30 °C for 2 h released all the phenolic acids ( p-coumaric, ferulic, and diferulic) but extracted only ~30% of...

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Bibliographic Details
Published in:Carbohydrate polymers 1995, Vol.26 (4), p.279-287
Main Authors: Saulnier, Luc, Marot, Cécile, Chanliaud, Elisabeth, Thibault, Jean-François
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cereal and baking product industries
Chemical Sciences
Food industries
Fundamental and applied biological sciences. Psychology
Polymers
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Summary:Sequential extractions with alkali have been carried out in order to study the nature of linkages which hold heteroxylans in maize bran cell walls. Treatment with 0.5 m sodium hydroxide at 30 °C for 2 h released all the phenolic acids ( p-coumaric, ferulic, and diferulic) but extracted only ~30% of heteroxylans (S 1); further treatment with 1.5 m potassium hydroxide at 100 °C for 2 h released the remaining heteroxylans (S 2). The heteroxylans from S 1 and S 2 had a similar neutral sugar composition and structure, but their weight average molecular weights were 270 kDa ( M w M n = 2 ) and 370 kDa ( M w M n = 2.8 ), respectively. Proteins (5%) are found with polysaccharides from S 1 and S 2, with different amino acid composition. The results suggest that covalent linkages through phenolic acids are only partly responsible for the associations of maize bran heteroxylans in the cell wall and that linkages to structural cell wall proteins were probably the main cause of heteroxylan insolubility.
ISSN:0144-8617
1879-1344
DOI:10.1016/0144-8617(95)00020-8