Chloroplast DNA variability in the genus Helianthus: restriction analysis and S1 nuclease mapping of DNA-DNA heteroduplexes

Chloroplast DNA (cpDNA) from 36 wild species of the genus Helianthus has been analysed with three restriction endonucleases (Bam HI, Hind III and Sst I). Out of the 71 restriction sites described on the reference cpDNA (sunflower cpDNA), three insertions/deletions and seven site modifications were d...

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Bibliographic Details
Published in:Plant molecular biology 1990-08, Vol.15 (2), p.269-280
Main Authors: Serror, P, Heyraud, F, Heizmann, P
Format: Article
Language:English
Subjects:
Base Sequence
Biochemistry, Molecular Biology
Biological and medical sciences
chemotaxonomy
chloroplast DNA
chloroplasts
Chloroplasts - chemistry
chromosome mapping
Classical genetics, quantitative genetics, hybrids
deletions
DNA
DNA - genetics
ecotypes
Fundamental and applied biological sciences. Psychology
genetic polymorphism
Genetics of eukaryotes. Biological and molecular evolution
genome
genomics
Helianthus
Helianthus annuus
Helianthus tuberosus
insertions
Life Sciences
Molecular Sequence Data
Nucleic Acid Hybridization
nucleotide sequences
Phylogeny
Plants - genetics
Polymorphism, Restriction Fragment Length
Pteridophyta, spermatophyta
Restriction Mapping
restriction site polymorphism
Single-Strand Specific DNA and RNA Endonucleases
Species Specificity
transcription (genetics)
Vegetals
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Summary:Chloroplast DNA (cpDNA) from 36 wild species of the genus Helianthus has been analysed with three restriction endonucleases (Bam HI, Hind III and Sst I). Out of the 71 restriction sites described on the reference cpDNA (sunflower cpDNA), three insertions/deletions and seven site modifications were detected during the survey of the other cpDNAs. Since restriction mapping showed only a very limited fraction of the DNA variability, we chose to adapt the S1 nuclease mapping technique to detect fine variations between chloroplast genomes. For this purpose, DNA-DNA heteroduplexes obtained between sunflower and wild-species DNAs were digested by S1 nuclease and the resulting mismatches were detected by classical endonuclease restriction and hybridization methods. The S1 nuclease mapping results were confirmed by sequencing one S1 nuclease-sensitive region detected between cultivated sunflower and two perennial wild-type species. As a result of these analyses, it appeared that the combination of restriction mapping and S1 nuclease mapping might be helpful to differentiate taxonomically close cytoplasms.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00036913