Nucleotide sequence of the 3'-terminal region of the RNA of the El Amar strain of plum pox potyvirus

The nucleotide sequence of the 3'-terminal 4773 nucleotides of the RNA of a widely divergent, aphid-transmissible strain of plum pox potyvirus isolated from Egypt (PPV-El Amar) was determined. The sequenced region covers the carboxy terminus of the cylindrical inclusion (CI) gene, and the putat...

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Bibliographic Details
Published in:Journal of general virology 1991, Vol.72 (7), p.1741-1746
Main Authors: Wetzel, T, Candresse, T, Ravelonandro, M, Delbos, R.P, Mazyad, H, Aboul-Ata, A.E, Dunez, J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
Biological and medical sciences
Capsid - genetics
Cloning, Molecular
coat proteins
DNA-Directed RNA Polymerases - genetics
embl/x56258
Endopeptidases
Fundamental and applied biological sciences. Psychology
Genetics
Life Sciences
Microbiology
Microbiology and Parasitology
Microcomputers
Molecular Sequence Data
nucleotide sequences
Open Reading Frames
Plant Viruses - genetics
Plum pox virus
Restriction Mapping
RNA
RNA, Viral - chemistry
Software
Viral Proteins - genetics
Virology
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Summary:The nucleotide sequence of the 3'-terminal 4773 nucleotides of the RNA of a widely divergent, aphid-transmissible strain of plum pox potyvirus isolated from Egypt (PPV-El Amar) was determined. The sequenced region covers the carboxy terminus of the cylindrical inclusion (CI) gene, and the putative 6K protein, the NIa protease, the NIb RNA polymerase and the coat protein genes, linked together as one large open reading frame (ORF) in a fashion similar to the canonical genomic organization of other potyviruses. The large ORF encoding the polyprotein is followed by a 217 nucleotide non-coding region and a poly(a) tail. However, whereas the three PPV strains previously sequenced show levels of identity in excess of 98%, PPV-El Amar shows levels of heterogeneity of 20% in the nucleotide sequence and 10% in the amino acid sequence, when compared with these previously sequenced strains. The N-terminal region of the capsid protein, postulated to be involved in the aphid transmission mechanism of the virus, was found to be the region which differed most between PPV-El Amar and the other strains.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-72-7-1741