New investigations of the structure of grape arabino-galactan-protein

The structure of arabinogalactan-protein (AGP) isolated from grape juice was studied by methylation analysis, n.m.r. spectroscopy, and interaction with peanut lectin, after specific degradation with purified enzymes and/or Smith degradation. AGP appeared to be homogeneous with a weight-average molec...

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Bibliographic Details
Published in:Carbohydrate research 1992, Vol.224, p.219-235
Main Authors: Saulnier, Luc, Brillouet, Jean-Marc, Moutounet, Michel, du Penhoat, Catherine Hervé, Michon, Véronique
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
arabinose
Biological and medical sciences
carbohydrate structure
Chemical Sciences
Fundamental and applied biological sciences. Psychology
galactose
Glycoproteins
grapes
Polymers
polysaccharides
Proteins
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Summary:The structure of arabinogalactan-protein (AGP) isolated from grape juice was studied by methylation analysis, n.m.r. spectroscopy, and interaction with peanut lectin, after specific degradation with purified enzymes and/or Smith degradation. AGP appeared to be homogeneous with a weight-average molecular weight of 110 000. Treatment of AGP with arabinofuranosidase release 88% of the arabinose and left GP 1. Hydrolysis of GP 1 with an endo-(1→6)-β- d-galactanase removed 50% of the galactose and left GP 2. Smith degradation of GP 1 gave a 3-linked galactan that still contained 3,6-linked residues. Endogalactanase- and Smith-degraded GP 1, but not AGP and GP 1, reacted strongly with peanut lectin. Thus, AGP is a 3-linked galactan cross-linked at positions 6. The core also carries, at positions 6, 6-linked galactan chains heavily 3-substituted with arabinofuranose residues.
ISSN:0008-6215
1873-426X
0008-6215
DOI:10.1016/0008-6215(92)84108-5