Characterization of the nuclear gene encoding chloroplast ribosomal protein S13 from Arabidopsis thaliana

A cDNA clone and a nuclear gene were characterised encoding the chloroplast 30s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloropla...

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Bibliographic Details
Published in:Current genetics 1995-09, Vol.28 (4), p.346-352
Main Authors: Kumar, R, Drouaud, J, Raynal, M, Small, J. (Institut National de Recherches Agronomiques, Versailles (France). Station de Genetique et d'Amelioration des Plantes)
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - chemistry
Arabidopsis - genetics
ARABIDOPSIS THALIANA
Base Sequence
Blotting, Northern
Blotting, Southern
Blotting, Western
Cell Nucleus - chemistry
Cell Nucleus - genetics
CHLOROPLASTE
Chloroplasts - chemistry
Chromosome Mapping
Cloning, Molecular
CLOROPLASTO
DNA, Complementary
Evolution, Molecular
GENE
GENES
Genes, Plant
Genetics
Life Sciences
Mitochondria - chemistry
Mitochondria - genetics
MITOCHONDRIE
MITOCONDRIA
Molecular Sequence Data
Molecular Weight
Phylogeny
PROTEINAS
PROTEINE
Ribosomal Proteins - analysis
Ribosomal Proteins - chemistry
RIBOSOMAS
RIBOSOME
Sequence Alignment
Sequence Analysis
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Summary:A cDNA clone and a nuclear gene were characterised encoding the chloroplast 30s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal polypeptide using antibodies raised against the polypeptide produced from part of the cDNA expressed in bacteria. The predicted amino-acid sequence contains an N-terminal extension which has several features characteristic of chloroplast transit peptides. Experiments suggest there is a single copy of this gene in A. thaliana and multiple copies in Brassica species. The origin of the mitochondrial S13 polypeptide in crucifers is also discussed.
ISSN:0172-8083
1432-0983
DOI:10.1007/bf00326432