Cellular localization of an embryonic interferon, ovine trophoblastin and its mRNA in sheep embryos during early pregnancy

The ovine embryo produces an interferon named ovine Trophoblastin (oTP) which is involved in the maternal recognition of pregnancy and ensures the maintenance of progesterone secretion by the corpus luteum. We have used indirect immunohistofluorescence and in situ hybridization on histological secti...

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Bibliographic Details
Published in:Biology of the cell 1990, Vol.68 (3), p.205-211
Main Authors: Guillomot, Michel, Michel, Catherine, Gaye, Pierre, Charlier, Nadia, Trojan, Jean, Martal, Jacques
Format: Article
Language:English
Subjects:
ANIMAL EMBRYOS
Animals
ARN MENSAJERO
ARN MESSAGER
EMBRIONES ANIMALES
Embryo, Mammalian - cytology
Embryo, Mammalian - metabolism
EMBRYON ANIMAL
embryonic interferon
Endometrium - cytology
Endometrium - metabolism
Female
Fluorescent Antibody Technique
GESTACION
GESTATION
IMMUNOFLUORESCENCE
immunohistofluorescence
in situ hybridization
INMUNOFLUORESCENCIA
INTERFERON
Interferon Type I
Interferons - genetics
Interferons - metabolism
Life Sciences
Luteolytic Agents - antagonists & inhibitors
MESSENGER RNA
Nucleic Acid Hybridization
OVIN
ovine trophoblastin (oTP)
OVINOS
PREGNANCY
Pregnancy Proteins - genetics
Pregnancy Proteins - metabolism
Pregnancy, Animal - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
SHEEP
Trophoblasts - cytology
Trophoblasts - metabolism
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Description
Summary:The ovine embryo produces an interferon named ovine Trophoblastin (oTP) which is involved in the maternal recognition of pregnancy and ensures the maintenance of progesterone secretion by the corpus luteum. We have used indirect immunohistofluorescence and in situ hybridization on histological sections to investigate the fate of this protein and its mRNA in ovine embryos from days 3 to 25 of pregnancy. The level of expression was measured by image analysis of the autoradiographs after in situ hybridization. Both techniques clearly demonstrated that oTP and its mRNA were specifically localized in the extra-embryonic trophoblast. Neither the embryonic cells, nor the yolk sac or the amniotic tissues produced the protein or its mRNA. The protein could be detected by d 11 of pregnancy in the elongated blastocyst. Maximum of expression is observed at d 14 and the level decreased by d 16 of pregnancy. The arrest of expression occurred in the regions of trophoblast which have established cellular contacts with the uterine epithelium during the implantation process.
ISSN:0248-4900
1768-322X
DOI:10.1016/0248-4900(90)90309-Q