The Expression Pattern of the Tonoplast Intrinsic Protein γ-TIP in Arabidopsis thaliana Is Correlated with Cell Enlargement

The vacuolar membrane (tonoplast) contains an abundant intrinsic protein with six membrane-spanning domains that is encoded by a small gene family. Different isoforms of tonoplast intrinsic protein (TIP) are expressed in different tissues or as a result of specific signals. Using promoter-β-glucuron...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-12, Vol.100 (4), p.1633-1639
Main Authors: Ludevid, Dolors, Höfte, Herman, Edward Himelblau, Chrispeels, Maarten J.
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Biological and medical sciences
Cell biochemistry
Cell growth
Cell physiology
Development and Growth Regulation
Embryos
Fundamental and applied biological sciences. Psychology
Genetics
Hypocotyls
In situ hybridization
Life Sciences
Messenger RNA
Plant physiology and development
Plant roots
Plants
Plants genetics
Seedlings
Stipules
Tonoplast
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Summary:The vacuolar membrane (tonoplast) contains an abundant intrinsic protein with six membrane-spanning domains that is encoded by a small gene family. Different isoforms of tonoplast intrinsic protein (TIP) are expressed in different tissues or as a result of specific signals. Using promoter-β-glucuronidase (GUS) fusions and in situ hybridization, we have examined the expression of γ-TIP in Arabidopsis thaliana. GUS staining of plants transformed with promoter-GUS fusions showed that γTIP gene expression is high in recently formed tissues of young roots. In the shoot, γ-TIP gene expression was highest in the vascular bundles of stems and petioles, as well as in the stipules and in the receptacle of the flower. No GUS activity was detected in root or shoot meristems or in older tissues, suggesting temporal control of γ-TIP gene expression associated with cell elongation and/or differentiation. In situ hybridization carried out with whole seedlings confirmed that in root tips, γ-TIP mRNA was present only in the zone of cell elongation just behind the apical meristem. In seedling shoots, mRNA abundance was also found to be correlated with cell expansion. These results indicate that γ-TIP may be expressed primarily at the time when the large central vacuoles are being formed during cell enlargement.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.100.4.1633