Effects of Lindane Exposure on Rainbow Trout (Oncorhynchus mykiss) Immunity: III. Effect on Nonspecific Immunity and B Lymphocyte Functions

• Published in: Ecotoxicology and environmental safety 1994-04, Vol.27 (3), p.324-334
• Main Authors: Dunier, M., Siwicki, A.K., Scholtens, J., Molin, S.D., Vergnet, C., Studnicka, M.
• Format: Article
• Language: English
• Subjects: Administration, Oral; Agnatha. Pisces; Animal, plant and microbial ecology; Animals; Applied ecology; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; Biological and medical sciences; Cell Division - drug effects; Ceruloplasmin - immunology; Ceruloplasmin - metabolism; Dose-Response Relationship, Drug; Drug Residues - analysis; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Fundamental and applied biological sciences. Psychology; Injections, Intraperitoneal; Kidney - drug effects; Kidney - immunology; Life Sciences; Lindane - administration & dosage; Lindane - pharmacokinetics; Lindane - toxicity; Lymphocyte Activation - drug effects; Muramidase - blood; Muramidase - immunology; Neutrophils - drug effects; Oncorhynchus mykiss - immunology; Phagocytosis - drug effects
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1006/eesa.1994.1026

The effect of the organochlorine insecticide lindane (γ-hexachlorocyclohexane) was examined on some major immune functions of rainbow trout (Oncorhynchus mykiss) on the chemiluminescent response of pronephric cells (PMA-induced) in phagocytosis, on the proliferation of lymphocytes with B and T mitogens, and on the number of B lymphocytes analyzed by cytofluorometry. Two different methods of exposure were tried via food (first protocol) and via a single intraperitoneal injection (second protocol). After the oral contamination at a daily body dose of 1 mg/kg for 30 days, a decreased chemiluminescent response was observed which persisted for two more weeks and disappeared over 1.5 months. No effect was observed on lymphocyte proliferation and on the number of circulating B lymphocytes. In the second protocol lindane was administered intraperitoneally at 10, 50, or 100 mg/kg body wt. After 45 days the lymphocyte proliferation of B cells was depressed but not the T cell one. The B cells number in head kidney as measured by cytofluorometry was not significantly modified. Some nonspecific immunity parameters in sera were significantly modified.