Electrochemical characterization of the artificial metalloenzyme papain-[(η6-arene)Ru(1,10-phenanthroline)Cl]

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2020-02, Vol.859, p.113882, Article 113882
• Main Authors: Nováková Lachmanová, Štěpánka, Pospíšil, Lubomír, Šebera, Jakub, Talbi, Barisa, Salmain, Michèle, Hromadová, Magdaléna
• Format: Article
• Language: English
• Subjects: Artificial metalloenzyme; Chemical Sciences; Coordination chemistry; Electrochemical admittance and impedance techniques; Organic chemistry; Papain; Ruthenium(II) complexes
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2020.113882

Electrochemical properties were studied for [(η6-arene)Ru(1,10-phenanthroline)Cl]Cl (arene = C6H5(CH2)2NHCOCH2Cl) organometallic complex 1, protein Papain PAP and its conjugate with organometallic complex 1-PAP. The latter can serve as an artificial metalloenzyme with catalytic activity in transfer hydrogenation. This work demonstrates that AC voltammetry and electrochemical impedance spectroscopy can be used as fast tools to screen the catalytic ability of 1-PAP electrochemically by studies of the catalytic hydrogen evolution reaction (HER). Proteins are known to catalyze this process, but we have shown that additional HER signal associated with the catalytic activity of 1 is observed for its conjugate with Papain 1-PAP. [Display omitted] •Electrochemical screening of the catalytic activity of an artificial metalloenzyme•Example of the papain-S-[(η6-arene)Ru(1,10-phenanthroline)Cl]+ conjugate•AC voltammetric determination of catalytic hydrogen evolution reaction