Development of a new TaqMan PCR assay for the detection of both Entamoeba gingivalis genotypes

Entamoeba gingivalis is a parasitic protozoan found in the mouth of patients suffering from periodontitis, a widespread oral disease with an underestimated prevalence and major consequences on health. We present the development of the first TaqMan PCR assay targeting both E. gingivalis subtypes. Thi...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2019-12, Vol.95 (4), p.114886-114886, Article 114886
Main Authors: Zaffino, Marie, Dubar, Marie, Debourgogne, Anne, Bisson, Catherine, Machouart, Marie
Format: Article
Language:English
Subjects:
Diagnostic
DNA, Protozoan - genetics
DNA, Ribosomal - genetics
Entamoeba - classification
Entamoeba - genetics
Entamoeba - isolation & purification
Entamoeba gingivalis
Entamoebiasis - parasitology
Genotype
Humans
Life Sciences
Microbiology and Parasitology
Molecular Diagnostic Techniques - methods
Molecular tools
Mycology
Parasitology
PCR
Periodontitis
Periodontitis - parasitology
Real-Time Polymerase Chain Reaction - methods
Reproducibility of Results
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Entamoeba gingivalis is a parasitic protozoan found in the mouth of patients suffering from periodontitis, a widespread oral disease with an underestimated prevalence and major consequences on health. We present the development of the first TaqMan PCR assay targeting both E. gingivalis subtypes. This method has been evaluated on 50 samples from patients diagnosed with periodontitis in comparison with 2 different conventional PCRs, and a real-time SYBR Green PCR. Fifty percent of the samples were found positive for the E. gingivalis ST1 subtype with this new PCR, the SYBR Green PCR and one of the conventional PCRs. Among the 25 remaining samples, 12 (24%) were found positive for the E. gingivalis ST2 kamaktlii variant. This new TaqMan PCR could be used before and after periodontitis treatment to follow its efficacy and measure the parasite load in order to better understand the role of these parasites in oral diseases.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2019.114886