Active mucus–cilia hydrodynamic coupling drives self-organization of human bronchial epithelium

• Published in: Nature physics 2020-11, Vol.16 (11), p.1158-1164
• Main Authors: Loiseau, Etienne, Gsell, Simon, Nommick, Aude, Jomard, Charline, Gras, Delphine, Chanez, Pascal, D’Ortona, Umberto, Kodjabachian, Laurent, Favier, Julien, Viallat, Annie
• Format: Article
• Language: English
• Subjects: 631/57; 639/301/923/966; 639/766/747; Atomic; Biological Physics; Classical and Continuum Physics; Complex Systems; Condensed Matter Physics; Epithelium; Mathematical and Computational Physics; Molecular; Mucus; Optical and Plasma Physics; Phase diagrams; Physics; Physics and Astronomy; Physiology; Respiratory diseases; Respiratory tract; Theoretical; Two dimensional models
• ISSN: 1745-2473; 1745-2481; 1476-4636
• DOI: 10.1038/s41567-020-0980-z

The respiratory tract is protected by mucus, a complex fluid transported along the epithelial surface by the coordinated beating of millions of microscopic cilia, hence the name of mucociliary clearance. Its impairment is associated with all severe chronic respiratory diseases. Yet, the relationship between ciliary density and the spatial scale of mucus transport, as well as the mechanisms that drive ciliary-beat orientations are much debated. Here, we show on polarized human bronchial epithelia that mucus swirls and circular orientational order of the underlying ciliary beats emerge and grow during ciliogenesis, until a macroscopic mucus transport is achieved for physiological ciliary densities. By establishing that the macroscopic ciliary-beat order is lost and recovered by removing and adding mucus, respectively, we demonstrate that cilia–mucus hydrodynamic interactions govern the collective dynamics of ciliary-beat directions. We propose a two-dimensional model that predicts a phase diagram of mucus transport in accordance with the experiments. This paves the way to a predictive in silico modelling of bronchial mucus transport in health and disease. The flow of fluid, such as mucus in the human respiratory tract, can affect biological function. Here the authors show that the hydrodynamic interactions mediated by mucus are essential for the directional coordination of ciliary beating in the lungs.