Cloning and expression analysis of a new hydrophobin cDNA from the ectomycorrhizal basidiomycete Pisolithus

Hydrophobins are fungal cell wall proteins which play a crucial role in cell adhesion and aggregative processes. We have identified a new hydrophobin cDNA (hydPt-3) in the symbiotic mycelium of Pisolithus tinctorius (putative P. albus) during the formation of ectomycorrhizae around eucalypt roots. T...

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Bibliographic Details
Published in:Current genetics 2001-07, Vol.39 (5-6), p.335-339
Main Authors: Duplessis, S, Sorin, C, Voiblet, C, Palin, B, Martin, F, Tagu, D
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Basidiomycota - genetics
Basidiomycota - growth & development
Biochemistry, Molecular Biology
DNA, Complementary - genetics
Fungal Proteins - genetics
Genetics
hydPt-1 gene
hydPt-2 gene
hydPt-3 gene
hydrophobin
Life Sciences
Molecular biology
Molecular Sequence Data
Pisolithus tinctorius
Plant Roots - microbiology
Plants genetics
Sequence Alignment
Sequence Homology, Amino Acid
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Summary:Hydrophobins are fungal cell wall proteins which play a crucial role in cell adhesion and aggregative processes. We have identified a new hydrophobin cDNA (hydPt-3) in the symbiotic mycelium of Pisolithus tinctorius (putative P. albus) during the formation of ectomycorrhizae around eucalypt roots. This sequence is highly divergent from two other previously identified Pisolithus symbiosis-regulated hydrophobins, hydPt-1 and hydPt-2. Also, expression analyses demonstrated that hydPt-3 is up-regulated during the formation of ectomycorrhizae. In contrast to phytopathogenic fungi, changes in glucose or ammonium concentrations in the growth medium did not influence the accumulation of any Pisolithus hydrophobin mRNAs. This suggests that other factors act as regulators of hydrophobin gene expression in ectomycorrhizae.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940100224