Flubendiamide, the first phthalic acid diamide insecticide, impairs neuronal calcium signalling in the honey bee’s antennae

[Display omitted] •External and internal calcium are involved in detection of pheromones in honey bee.•Insecticide flubendiamide disturbs intracellular calcium homeostasis.•In vitro and calcium imaging assays are tools for insecticides risk assessment. Calcium is an important intracellular second me...

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Bibliographic Details
Published in:Journal of insect physiology 2020-08, Vol.125, p.104086, Article 104086
Main Authors: Kadala, Aklesso, Charreton, Mercédès, Collet, Claude
Format: Article
Language:English
Subjects:
Animal biology
Animals
Arthropod Antennae - drug effects
Arthropod Antennae - physiology
Benzamides - adverse effects
Caffeine
Calcium
Calcium - metabolism
Cellular Biology
Flubendiamide
Honey bee antennal neurons
Insecticides - adverse effects
Life Sciences
Neurons - drug effects
Neurons - physiology
Pheromone
Ryanodine
Signal Transduction - drug effects
Sulfones - adverse effects
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Summary:[Display omitted] •External and internal calcium are involved in detection of pheromones in honey bee.•Insecticide flubendiamide disturbs intracellular calcium homeostasis.•In vitro and calcium imaging assays are tools for insecticides risk assessment. Calcium is an important intracellular second messenger involved in several processes such as the transduction of odour signals and neuronal excitability. Despite this critical role, relatively little information is available with respect to the impact of insecticides on the dynamics of intracellular calcium homeostasis in olfactory neurons. For the first time here, physiological stimuli (depolarizing current or pheromone) were shown to elicit calcium transients in peripheral neurons from the honey bee antenna. In addition, neurotoxic xenobiotics (the first synthetic phthalic diamide insecticide flubendiamide or botanical alkaloids ryanodine and caffeine) do interfere with normal calcium homeostasis. Our in vitro experiments show that these three xenobiotics can induce sustained abnormal calcium transients in antennal neurons. The present results provide a new insight into the toxicity of diamides, showing that flubendiamide drastically impairs calcium homeostasis in antennal neurons. We propose that a calcium imaging assay should provide an efficient tool dedicated to the modern assessment strategies of insecticides toxicity.
ISSN:0022-1910
1879-1611
DOI:10.1016/j.jinsphys.2020.104086