An Adenovirus Vector for Gene Transfer into Neurons and Glia in the Brain

The efficient introduction of genetic material into quiescent nerve cells is important in the study of brain function and for gene therapy of neurological disorders. A replication-deficient adenoviral vector that contained a reporter gene encoding β-galactosidase infected rat nerve cells in vitro an...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1993-02, Vol.259 (5097), p.988-990
Main Authors: G. Le Gal La Salle, Robert, J. J., Berrard, S., Ridoux, V., Stratford-Perricaudet, L. D., Perricaudet, M., Mallet, J.
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
adenovirus
Adenoviruses
Animals
Antibodies
Astrocytes - metabolism
Astrocytes - microbiology
Avian Sarcoma Viruses - genetics
beta-Galactosidase - genetics
Biological and medical sciences
Biotechnology
Brain - cytology
Cultured cells
DNA - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression
Genetic engineering
Genetic technics
Genetic Vectors
Hippocampus
Hippocampus - cytology
Hippocampus - metabolism
Inoculation
Life Sciences
Methods. Procedures. Technologies
Neuroglia
Neuroglia - metabolism
Neuroglia - microbiology
Neurons
Neurons - metabolism
Neurons - microbiology
Neurons and Cognition
Promoter Regions, Genetic - genetics
Pyramidal cells
Rats
Substantia nigra
Substantia Nigra - cytology
Substantia Nigra - metabolism
Transfection
Vectors (cloning, transfer, expression). Insertion sequences and transposons
Viruses
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Summary:The efficient introduction of genetic material into quiescent nerve cells is important in the study of brain function and for gene therapy of neurological disorders. A replication-deficient adenoviral vector that contained a reporter gene encoding β-galactosidase infected rat nerve cells in vitro and in vivo. β-Galactosidase was expressed in almost all sympathetic neurons and astrocytes in culture. After stereotactic inoculations into the rat hippocampus and the substantia nigra, β -galactosidase activity was detected for 2 months. Infected cells were identified as microglial cells, astrocytes, or neurons with anatomical, morphological, and immunohistochemical criteria. No obvious cytopathic effect was observed.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.8382374