Development of twelve novel polymorphic microsatellite DNA markers for the Boeseman’s rainbowfish (Melanotaenia boesemani) and tests for their cross-utility in 21 rainbowfish species from West Papua (Indonesia)

We developed 12 microsatellite markers for the endangered Boeseman’s rainbowfish (Melanotaenia boesemani). Twenty-eight individuals from the type locality at Ayamaru Lake were examined, and all loci were polymorphic with a number of alleles per locus varying from 3 to 18. Average observed and expect...

Full description

Saved in:
Bibliographic Details
Published in:European journal of wildlife research 2014-12, Vol.60 (6), p.941-946
Main Authors: Nugraha, Media Fitri Isma, Pouyaud, Laurent, Carman, Odang, Kadarusman, Widyastuti, Utut, Avarre, Jean-Christophe
Format: Article
Language:English
Subjects:
Animal biology
Biomedical and Life Sciences
Deoxyribonucleic acid
DNA
Ecology
Endangered & extinct species
Endangered species
Fish
Fish & Wildlife Biology & Management
Genetic markers
genotyping
Inbreeding
inbreeding coefficient
lakes
Life Sciences
loci
Melanotaenia
microsatellite repeats
null alleles
Polymorphism
Population studies
Technical Notes
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We developed 12 microsatellite markers for the endangered Boeseman’s rainbowfish (Melanotaenia boesemani). Twenty-eight individuals from the type locality at Ayamaru Lake were examined, and all loci were polymorphic with a number of alleles per locus varying from 3 to 18. Average observed and expected heterozygosities were 0.681 and 0.678, respectively. Cross-species amplification was successfully obtained for 21 Melanotaenia species, with a number of alleles per locus ranging between 1 and 20. Average observed and expected heterozygosities varied between 0.105 and 0.708 and 0.118–0.755, respectively. Only 21 inbreeding coefficient (Fis) values presented a significant homozygote excess among the 264 locus-by-locus calculated values. Tests for genotyping errors revealed that four of these 21 significant Fis values could be explained by the presence of null alleles. These new microsatellite markers appear highly reliable for further conservation purposes or population genetic studies of the many rainbowfish endangered species.
ISSN:1612-4642
1439-0574
DOI:10.1007/s10344-014-0868-2