Efficiency of benthic diatom-associated bacteria in the removal of benzo(a)pyrene and fluoranthene

• Published in: The Science of the total environment 2021-01, Vol.751, p.141399-141399, Article 141399
• Main Authors: Kahla, Oumayma, Melliti Ben Garali, Sondes, Karray, Fatma, Ben Abdallah, Manel, Kallel, Najwa, Mhiri, Najla, Zaghden, Hatem, Barhoumi, Badreddine, Pringault, Olivier, Quéméneur, Marianne, Tedetti, Marc, Sayadi, Sami, Sakka Hlaili, Asma
• Format: Article
• Language: English
• Subjects: Bizerte Lagoon; Hydrocarbon-degrading bacteria; Nitzschia sp. phycosphere; Ocean, Atmosphere; PAH accumulation; PAH biodegradation; Sciences of the Universe
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2020.141399

We investigated the efficiency of a benthic diatom-associated bacteria in removing benzo(a)pyrene (BaP) and fluoranthene (Flt). The diatom, isolated from a PAH-contaminated sediment of the Bizerte Lagoon (Tunisia), was exposed in axenic and non-axenic cultures to PAHs over 7 days. The diversity of the associated bacteria, both attached (AB) and free-living bacteria (FB), was analyzed by the 16S rRNA amplicon sequencing. The diatom, which maintained continuous growth under PAH treatments, was able to accumulate BaP and Flt, with different efficiencies between axenic and non-axenic cultures. Biodegradation, which constituted the main process for PAH elimination, was enhanced in the presence of bacteria, indicating the co-metabolic synergy of microalgae and associated bacteria in removing BaP and Flt. Diatom and bacteria showed different capacities in the degradation of BaP and Flt. Nitzschia sp. harbored bacterial communities with a distinct composition between attached and free-living bacteria. The AB fraction exhibited higher diversity and abundance relative to FB, while the FB fraction contained genera with the known ability of PAH degradation, such as Marivita, Erythrobacter, and Alcaligenes. Moreover, strains of Staphylococcus and Micrococcus, isolated from the FB community, showed the capacity to grow in the presence of crude oil. These results suggest that a “benthic Nitzschia sp.-associated hydrocarbon-degrading bacteria” consortium can be applied in the bioremediation of PAH-contaminated sites. [Display omitted] •An indigenous benthic diatom accumulated BaP and Flt, but biodegradation played the main role in the removal of PAH.•The biodegradation was enhanced by the presence of the diatom and its associated bacteria.•The diatom harbored bacterial genera identified as potential PAH degraders.•Strains isolated from the associated bacteria were able to grow in the presence of crude oil.