Second-Generation Dendrimers with Chondroitin Sulfate Type‑E Disaccharides as Multivalent Ligands for Langerin

Chondroitin sulfate type-E (CS-E) is a sulfated polysaccharide that shows several interesting biological activities, such as modulation of the neuronal growth factor signaling and its interaction with langerin, a C-type lectin with a crucial role in the immunological system. However, applications of...

Full description

Saved in:
Bibliographic Details
Published in:Biomacromolecules 2020-07, Vol.21 (7), p.2726-2734
Main Authors: Domínguez-Rodríguez, Pedro, Vivès, Corinne, Thepaut, Michel, Fieschi, Franck, Nieto, Pedro M, de Paz, José L, Rojo, Javier
Format: Article
Language:English
Subjects:
Biochemistry, Molecular Biology
Life Sciences
Structural Biology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chondroitin sulfate type-E (CS-E) is a sulfated polysaccharide that shows several interesting biological activities, such as modulation of the neuronal growth factor signaling and its interaction with langerin, a C-type lectin with a crucial role in the immunological system. However, applications of CS-E are hampered by the typical heterogeneous structure of the natural polysaccharide. Well-defined, homogeneous CS-E analogues are highly demanded. Here, we report the synthesis of monodispersed, structurally well-defined second-generation glycodendrimers displaying up to 18 CS-E disaccharide units. These complex multivalent systems have a molecular weight and a number of disaccharide repeating units comparable with those of the natural polysaccharides. In addition, surface plasmon resonance experiments revealed a calcium-independent interaction between these glycodendrimers and langerin, in the micromolar range, highlighting the utility of these compounds as CS-E mimetics.
ISSN:1525-7797
1526-4602
DOI:10.1021/acs.biomac.0c00476