Chromatographic separation simulation of metal‐chelating peptides from surface plasmon resonance binding parameters

Some metal‐chelating peptides have antioxidant properties, with potential nutrition, health, and cosmetics applications. This study aimed to simulate their separation on immobilized metal ion affinity chromatography from their affinity constant for immobilized metal ion determined in surface plasmon...

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Bibliographic Details
Published in:Journal of separation science 2020-06, Vol.43 (11), p.2031-2041
Main Authors: Muhr, Laurence, Pontvianne, Steve, Selmeczi, Katalin, Paris, Cédric, Boschi‐Muller, Sandrine, Canabady‐Rochelle, Laetitia
Format: Article
Language:English
Subjects:
Adsorption
Affinity
Antioxidants
Chelating Agents - chemistry
Chelating Agents - isolation & purification
Chelation
Chemical Sciences
Chromatography
Chromatography, Affinity
Computer simulation
Cosmetics
Engineering Sciences
immobilized metal ion affinity chromatography
Ions
Mathematical models
Metal ions
modeling
Nutrition
Peptides
Peptides - chemistry
Peptides - isolation & purification
Separation
Simulation
Surface Plasmon Resonance
Transition Elements - chemistry
Transition Elements - isolation & purification
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Summary:Some metal‐chelating peptides have antioxidant properties, with potential nutrition, health, and cosmetics applications. This study aimed to simulate their separation on immobilized metal ion affinity chromatography from their affinity constant for immobilized metal ion determined in surface plasmon resonance, both technics are based on peptide‐metal ion interactions. In our approach, first, the affinity constant of synthetic peptides was determined by surface plasmon resonance and used as input data to numerically simulate the chromatographic separation with a transport‐dispersive model based on Langmuir adsorption isotherm. Then, chromatographic separation was applied on the same peptides to determine their retention time and compare this experimental tR with the simulated tR obtained from simulation from surface plasmon resonance data. For the investigated peptides, the relative values of tR were comparable. Hence, our study demonstrated the pertinence of such numerical simulation correlating immobilized metal ion affinity chromatography and surface plasmon resonance.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201900882