Food-dependent set-up of the DiDGI® dynamic in vitro system: Correlation with the porcine model for protein digestion of soya-based food

•Food-dependent programme was developed for an in vitro dynamic model of digestion.•Apparent digestibility of nitrogen was consistent in vitro and in vivo.•Both models yielded similar molecular weight distributions of gastric peptides.•Duodenal proteolysis was globally comparable between models.•The...

Full description

Saved in:
Bibliographic Details
Published in:Food chemistry 2021-03, Vol.341 (Pt 2), p.128276-128276, Article 128276
Main Authors: Reynaud, Yohan, Couvent, Angélique, Manach, Aline, Forest, David, Lopez, Michel, Picque, Daniel, Souchon, Isabelle, Rémond, Didier, Dupont, Didier
Format: Article
Language:English
Subjects:
Animals
Digestion
Duodenum - metabolism
Food and Nutrition
Gastric Emptying
Glycine max - metabolism
Ileum - metabolism
In Vitro Techniques
Intestinal transit
Jejunum - metabolism
Life Sciences
Milk Proteins - metabolism
Mini-pig
Models, Biological
Nitrogen - metabolism
Plant Proteins, Dietary - metabolism
Proteolysis
Soya milk
Stomach
Swine
Tofu
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Food-dependent programme was developed for an in vitro dynamic model of digestion.•Apparent digestibility of nitrogen was consistent in vitro and in vivo.•Both models yielded similar molecular weight distributions of gastric peptides.•Duodenal proteolysis was globally comparable between models.•The DiDGI® demonstrated less inter-trial variability for measured parameters. The present study compared in vivo protein digestion in a miniature pig model with the dynamic in vitro system DiDGI®, using three digestive compartments (stomach, duodenum, and jejunum + ileum). Two soya-based meals—commercial soya milk and tofu—were studied, each with the same macronutrient content but different macrostructures. Our aim was to first deduce from the in vivo experiments in pigs key digestive parameters such as gastric pH, stomach emptying kinetics, and intestinal transit time, in order to design a relevant set-up for the dynamic in vitro system. Then, we compared digestive samples collected at fixed sampling times from both in vivo and in vitro models regarding different values related to proteolysis. We observed similar evolutions of gastric peptide distribution and duodenal proteolysis between models. Overall, apparent ileal digestibility of nitrogen was similar in vitro and in vivo and the differences between the two meals were conserved between models.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2020.128276