Investigation of the Action Patterns of Pectinmethylesterase Isoforms through Kinetic Analyses and NMR Spectroscopy

• Published in: The Journal of biological chemistry 1998-12, Vol.273 (50), p.33150-33156
• Main Authors: Catoire, Laurent, Pierron, Monique, Morvan, Claudine, du Penhoat, Catherine Hervé, Goldberg, Renée
• Format: Article
• Language: English
• Subjects: Biochemistry, Molecular Biology; Biophysics; Life Sciences
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.273.50.33150

Well characterized pectin samples were incubated with cell wall-bound and -solubilized pure isoforms of pectinmethylesterase from mung bean hypocotyls ( Vigna radiata ). Both enzyme activity and average product structure were determined at intervals along the deesterification pathway at pH 5.6 and 7.6. The latter analyses were performed by 13 C NMR spectroscopy, and the degree of esterification was probed by both 13 C NMR and potentiometric measurements. A dichotomy was observed in the behavior of the α and γ isoforms when compared with that of the β isoenzyme. Ideal blockwise deesterification mechanisms reproduced the experimental average structures (methylester distribution) throughout the course of the reaction. In the case of the α and γ isoforms, a single chain mechanism associated with a free carboxyl group at the second nearest neighbor position could be postulated at pH 5.6, whereas some multiple attack character was required to reproduce the data at pH 7.6. Several mechanisms that differed from the preceding ones were compatible with the data for the β isoform at the two pH values. Both the nature of the polysaccharides produced in these reactions and the role of pectinmethylesterase in the cell wall-stiffening process along the growth gradient are discussed.