Conjugal transfer to the determinants for bacteriocin (lacticin 481) production and immunity in Lactococcus lactis subsp. lactis CNRZ 481

The lacticin 481-producer (Lct+), L. lactis subsp. lactis (L. lactis) CNRZ 481 harbours 5 plasmids of 6.5, 7.5, 20, 37 and 69 kb. Novobiocin treatment of L. lactis 481 led to the appearance of lacticin 481 deficient variants which had all lost the 69 kb plasmid. Conjugal transfer of the lacticin 481...

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Bibliographic Details
Published in:FEMS microbiology letters 1993-09, Vol.112 (3), p.313-318
Main Authors: Piard, J.C, Delorme, C, Novel, M, Desmazeaud, M, Novel, G
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Bacterial Proteins - genetics
Bacterial Proteins - pharmacology
Bacteriocins
Bacteriology
Base Sequence
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Chromosome Mapping
Conjugation, Genetic
drug resistance
Drug Resistance, Microbial
Fundamental and applied biological sciences. Psychology
gene dosage
gene transfer
Genes, Bacterial
Genetics
Industrial strains
Lactococcus lactis
Lactococcus lactis - drug effects
Lactococcus lactis - genetics
Lactococcus lactis subsp. lactis
Life Sciences
Microbiology and Parasitology
Mission oriented research
Molecular Sequence Data
Mutation
Novobiocin
Novobiocin - pharmacology
phenotype
Plasmid
plasmids
Plasmids - genetics
structural genes
Transconjugants
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Description
Summary:The lacticin 481-producer (Lct+), L. lactis subsp. lactis (L. lactis) CNRZ 481 harbours 5 plasmids of 6.5, 7.5, 20, 37 and 69 kb. Novobiocin treatment of L. lactis 481 led to the appearance of lacticin 481 deficient variants which had all lost the 69 kb plasmid. Conjugal transfer of the lacticin 481 structural gene (lct) into the plasmid free strain L. lactis IL1441 yielded Lct+ transconjugants at a 10(-4) frequency, which carried a plasmid with an apparent size of 120-130 kb. Southern hybridization analyses showed that the lct gene was located on the 69 kb plasmid in L. lactis 481 and on the 120-130 kb plasmid in the transconjugants. The lct gene was in higher copy number in transconjugants than in the parental strain resulting in two-fold higher lacticin 481 production in the former strain.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1993.tb06468.x