Hyperpolarized NMR Metabolomics at Natural 13 C Abundance

Metabolomics plays a pivotal role in systems biology, and NMR is a central tool with high precision and exceptional resolution of chemical information. Most NMR metabolomic studies are based on H 1D spectroscopy, severely limited by peak overlap. C NMR benefits from a larger signal dispersion but is...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2020-11, Vol.92 (22), p.14867-14871
Main Authors: Dey, Arnab, Charrier, Benoît, Martineau, Estelle, Deborde, Catherine, Gandriau, Elodie, Moing, Annick, Jacob, Daniel, Eshchenko, Dmitry, Schnell, Marc, Melzi, Roberto, Kurzbach, Dennis, Ceillier, Morgan, Chappuis, Quentin, Cousin, Samuel F, Kempf, James G, Jannin, Sami, Dumez, Jean-Nicolas, Giraudeau, Patrick
Format: Article
Language:English
Subjects:
Carbon Isotopes - analysis
Carbon Isotopes - chemistry
Life Sciences
Magnetic Resonance Spectroscopy
Metabolomics - methods
Vegetal Biology
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Summary:Metabolomics plays a pivotal role in systems biology, and NMR is a central tool with high precision and exceptional resolution of chemical information. Most NMR metabolomic studies are based on H 1D spectroscopy, severely limited by peak overlap. C NMR benefits from a larger signal dispersion but is barely used in metabolomics due to ca. 6000-fold lower sensitivity. We introduce a new approach, based on hyperpolarized C NMR at natural abundance, that circumvents this limitation. A new untargeted NMR-based metabolomic workflow based on dissolution dynamic nuclear polarization (d-DNP) for the first time enabled hyperpolarized natural abundance C metabolomics. Statistical analysis of resulting hyperpolarized C data distinguishes two groups of plant (tomato) extracts and highlights biomarkers, in full agreement with previous results on the same biological model. We also optimize parameters of the semiautomated d-DNP system suitable for high-throughput studies.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.0c03510