Use of date products in production of the thermophilic dairy starter strain Streptococcus thermophilus

Date-coat sugar extract and date-seed hydrolysate were utilized as the main constituents of a medium for the production of a thermophilic dairy starter strain. Date-coat sugar extract was used as the carbon source, while date-seed hydrolysate was used as the nitrogen source. A suitable concentration...

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Bibliographic Details
Published in:Bioresource technology 1999-03, Vol.67 (3), p.291-295
Main Authors: Nancib, Nabil, Ghoul, Mostefa, Larous, Larbi, Nancib, Aïcha, Adimi, Leila-zed, Remmal, Mouloud, Boudrant, Joseph
Format: Article
Language:English
Subjects:
Amino acids
Bacteria
Biological and medical sciences
Biomass
Biotechnology
Cell culture
Culture
Dairy products
Date products
Engineering Sciences
Food industries
Food processing
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Lactic acid
Streptococcus thermophilus
Use and upgrading of agricultural and food by-products. Biotechnology
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Summary:Date-coat sugar extract and date-seed hydrolysate were utilized as the main constituents of a medium for the production of a thermophilic dairy starter strain. Date-coat sugar extract was used as the carbon source, while date-seed hydrolysate was used as the nitrogen source. A suitable concentration of date-coat sugar was in the range of 50 mg sugar/ml. Addition of various amounts of date-seed hydrolysate as the sole nitrogen source in the medium showed that, in spite of a nitrogen insufficiency found in the hydrolysate, the production of the starter strain increased with date-seed hydrolysate (nitrogen) concentration, but the maximum production of biomass was less than that observed with other nitrogen sources. Therefore, various amounts of urea were added and a mixture of urea (6 mg/ml) and of date-seed hydrolysate (4.0 mg/ml) allowed an increase in the concentration of the biomass. The addition of date-seed ash as a mineral source, at a concentration of 1.0 mg/ml in the medium containing date-coat sugar extract, date-seed hydrolysate, and urea could substitute for MgSO 4, and MnSO 4 of the usual medium. This medium gave the maximum production of the thermophilic lactic acid bacteria (0.57 mg/ml) and lactic acid (2 mg/ml), very close to what was obtained with the Elliker broth medium.
ISSN:0960-8524
1873-2976
DOI:10.1016/S0960-8524(98)00115-1