Influence of the microstructure and mechanical strength of nanofibers of biodegradable polymers with hydroxyapatite in stem cells growth. Electrospinning, characterization and cell viability

Nanofibers of poly (lactic acid) (PLA) and poly (caprolactone) (PCL) with different amounts of nanohydroxyapatite (nHA) were produced by electrospinning, changing both the applied voltage and collector's rotation. Characterization of the nanofibers by scanning and transmission electron microsco...

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Bibliographic Details
Published in:Polymer degradation and stability 2012-10, Vol.97 (10), p.2037-2051
Main Authors: Ribeiro Neto, Wilson A., Pereira, Ildeu H.L., Ayres, Eliane, de Paula, Ana C.C., Averous, Luc, Góes, Alfredo M., Oréfice, Rodrigo L., Suman Bretas, Rosario Elida
Format: Article
Language:English
Subjects:
Applied sciences
biodegradability
Bionanocomposites
Biopolymers
cell growth
Cell viability
Chemical Sciences
crystal structure
differential scanning calorimetry
Electrospinning
Exact sciences and technology
Fourier transform infrared spectroscopy
hydroxyapatite
microstructure
modulus of elasticity
nanofibers
Nanohydroxyapatite
Physicochemistry of polymers
polylactic acid
Polymer industry, paints, wood
Polymers
scanning electron microscopy
Stem cell
stem cells
Technology of polymers
temperature
thermogravimetry
transmission electron microscopy
X-radiation
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Summary:Nanofibers of poly (lactic acid) (PLA) and poly (caprolactone) (PCL) with different amounts of nanohydroxyapatite (nHA) were produced by electrospinning, changing both the applied voltage and collector's rotation. Characterization of the nanofibers by scanning and transmission electron microscopy (SEM, TEM), wide angle X-rays diffraction (WAXD), infrared (FTIR), differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA) and dynamic mechanical analysis (DMTA) showed that the PLA/nHA fibers had larger diameters, lower crystallinities, lower degradation temperatures and higher elastic modulus than the PCL/nHA nanofibers. The crystalline phases of the PLA/nHA fibers were a mixture of α and α′ phases, while the crystalline phase of the PCL/nHA samples was mainly α phase. In both set of mats, the highest amount of viable cells with early osteogenic activity after 7 days was obtained with the mats with the highest amount of nHA (5 wt%), the lowest amount of crystallinity and the highest elastic modulus.
ISSN:0141-3910
1873-2321
DOI:10.1016/j.polymdegradstab.2012.03.048