In situ isobaric lipid mapping by MALDI–ion mobility separation–mass spectrometry imaging

The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustacean Gammarus fossarum using matrix‐assisted laser desorption/ionization (MALDI)...

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Bibliographic Details
Published in:Journal of mass spectrometry. 2020-09, Vol.55 (9), p.e4531-n/a
Main Authors: Fu, Tingting, Oetjen, Janina, Chapelle, Manuel, Verdu, Alexandre, Szesny, Matthias, Chaumot, Arnaud, Degli‐Esposti, Davide, Geffard, Olivier, Clément, Yohann, Salvador, Arnaud, Ayciriex, Sophie
Format: Article
Language:English
Subjects:
Amphipoda - chemistry
Animals
Aquatic crustaceans
Chemical Sciences
Crustaceans
Dimensions
Environmental Sciences
Female
Freshwater
Freshwater crustaceans
Imaging techniques
Inland water environment
ion mobility separation
Ion Mobility Spectrometry - methods
Ionic mobility
Ionization
Ions
Isomerism
Lasers
lipid isomers
Lipids
Lipids - analysis
Lipids - chemistry
MALDI
Mapping
Mass spectrometry
mass spectrometry imaging
Mass spectroscopy
Mobility
Molecular Structure
Scientific imaging
Separation
Spatial distribution
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectroscopy
Tandem Mass Spectrometry
TIMS
Tissues
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Summary:The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustacean Gammarus fossarum using matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in combination with an additional separation dimension using ion mobility spectrometry (IMS). The high‐resolution trapped ion mobility spectrometry (TIMS) allowed efficient separation of isobaric/isomeric lipids showing distinct spatial distributions. The structures of the lipids were further characterized by MS/MS analysis. It is demonstrated that MALDI MSI with mobility separation is a powerful tool for distinguishing and localizing isobaric/isomeric lipids.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.4531